MYH9 Recombinant antibody
MYH9 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
3E1
验证数据展示
经过测试的应用
Positive WB detected in | U-251 cells, U-87 MG cells, HeLa cells, A431 cells, U2OS cells, human placenta tissue, rat kidney tissue |
Positive IHC detected in | rat kidney tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:4950-1:19800 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
81204-1-RR targets MYH9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | MYH9 fusion protein Ag1612 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | myosin, heavy chain 9, non-muscle |
Synonyms | NMMHC II-a, Myosin-9, Myosin heavy chain, non-muscle IIa, Myosin heavy chain 9, Myosin 9 |
Calculated Molecular Weight | 227 kDa |
Observed Molecular Weight | 224-227 kDa |
GenBank Accession Number | BC011915 |
Gene Symbol | MYH9 |
Gene ID (NCBI) | 4627 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P35579 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for MYH9 antibody 81204-1-RR | Download protocol |
IHC protocol for MYH9 antibody 81204-1-RR | Download protocol |
IF protocol for MYH9 antibody 81204-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |