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CoraLite®594-conjugated MYL3 Monoclonal antibody

MYL3 Monoclonal Antibody for IF/ICC, IF-P

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF/ICC, IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2E4B2

Cat no : CL594-66286

Print datasheet

Synonyms

Myosin light chain 3, Myosin light chain 1, slow-twitch muscle B/ventricular isoform, MYL 3, MLC-lV/sb, MLC1V

验证数据展示

  • IF Staining of C2C12 using CL594-66286

    IF Staining of C2C12 using CL594-66286

    Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cells using CL594-66286 (MYL3 antibody) at dilution of 1:100.

  • IF Staining of mouse skeletal muscle using CL594-66286

    IF Staining of mouse skeletal muscle using CL594-66286

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using CL594-66286 (MYL3 antibody) at dilution of 1:100.

经过测试的应用

Positive IF-P detected inmouse skeletal muscle tissue
Positive IF/ICC detected inC2C12 cells
Planning an IHC experiment? We recommend our IHCeasy MYL3 Ready-To-Use IHC Kit. MYL3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66286-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66286 targets MYL3 in IF/ICC, IF-P applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications IF/ICC, IF-P Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen MYL3 fusion protein Ag24535 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name myosin, light chain 3, alkali; ventricular, skeletal, slow
Synonyms Myosin light chain 3, Myosin light chain 1, slow-twitch muscle B/ventricular isoform, MYL 3, MLC-lV/sb, MLC1V
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 22-25 kDa
GenBank Accession NumberBC009790
Gene Symbol MYL3
Gene ID (NCBI) 4634
RRIDAB_2883551
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP08590
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYL3, also named as MLC1v, is an essential light chain of myosin that is associated with muscle contraction. It is expressed in ventricular and slow skeletal muscle. MYL3 may serve as a target for caspase-3 in dying cardiomyocytes. Mutations of MYL3 gene cause hypertrophic cardiomyopathy. MYL3 has been identified as potential serum biomarker for drug induced myotoxicity. Great increase in MYL3 serum concentration has been observed in rats with cardiac and skeletal muscle injury. (PMID:21685905)

实验方案

Product Specific Protocols
IF protocol for CL594 MYL3 antibody CL594-66286Download protocol
Standard Protocols
Click here to view our Standard Protocols