验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue, rat heart tissue |
| Positive IHC detected in | mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy MYL7 Ready-To-Use IHC Kit. MYL7 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
81570-1-RR targets MYL7 in WB, IHC, ELISA applications and shows reactivity with mouse, rat, human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 经测试反应性 | mouse, rat, human |
| 免疫原 | MYL7 fusion protein Ag11058 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | myosin, light chain 7, regulatory |
| 别名 | MLC 2a, MLC2a, MYL2A, MYL7, MYLC2A, Myosin light chain 2a |
| 计算分子量 | 175 aa, 19 kDa |
| 观测分子量 | 19 kDa |
| GenBank蛋白编号 | BC027915 |
| 基因名称 | MYL7 |
| Gene ID (NCBI) | 58498 |
| RRID | AB_2935563 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q01449 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MYL7, also known as myosin light chain 2a (MLC2a), is essential for heart development. The expression of MYL7 in heart is predominatantly restricted to the atrium. So its expression has been considered as useful marker for atrial cardiomyocytes.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MYL7 antibody 81570-1-RR | Download protocol |
| IHC protocol for MYL7 antibody 81570-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |