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验证数据展示

  • WB analysis using 81570-1-RR

    WB analysis using 81570-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81570-1-RR (MYL7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 81570-1-RR

    IHC staining of mouse heart using 81570-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 81570-1-RR (MYL7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYL7 antibody included
  • Affinity and Kinetic Characterization of 81570-1-RR

    Affinity and Kinetic Characterization of 81570-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81570-1-RR against Human MYL7 were performed. The affinity constant is 0.25 nM.

MYL7 Recombinant antibody

MYL7 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 81570-1-RR

Print datasheet

CloneNo. 2C5

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat, human

Applications

WB, IHC, ELISA

Synonyms

MLC 2a, MLC2a, MYL2A, MYL7, MYLC2A, Myosin light chain 2a

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 750
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse heart tissue, rat heart tissue
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy MYL7 Ready-To-Use IHC Kit. MYL7 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81570-1-RR targets MYL7 in WB, IHC, ELISA applications and shows reactivity with mouse, rat, human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity mouse, rat, human
Immunogen MYL7 fusion protein Ag11058 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name myosin, light chain 7, regulatory
Synonyms MLC 2a, MLC2a, MYL2A, MYL7, MYLC2A, Myosin light chain 2a
Calculated Molecular Weight 175 aa, 19 kDa
Observed Molecular Weight19 kDa
GenBank Accession NumberBC027915
Gene Symbol MYL7
Gene ID (NCBI) 58498
RRIDAB_2935563
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ01449
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYL7, also known as myosin light chain 2a (MLC2a), is essential for heart development. The expression of MYL7 in heart is predominatantly restricted to the atrium. So its expression has been considered as useful marker for atrial cardiomyocytes.

实验方案

Product Specific Protocols
WB protocol for MYL7 antibody 81570-1-RRDownload protocol
IHC protocol for MYL7 antibody 81570-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols