验证数据展示
经过测试的应用
| Positive WB detected in | rat heart tissue, mouse heart tissue |
| Positive FC (Intra) detected in | C2C12 cells |
Planning an IHC experiment? We recommend our IHCeasy MYL7 Ready-To-Use IHC Kit. MYL7 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 81570-1-PBS
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-81570 targets MYL7 in WB, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, FC (Intra) Application Description |
| Tested Reactivity | human, mouse, rat |
| Immunogen | MYL7 fusion protein Ag11058 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | myosin, light chain 7, regulatory |
| Synonyms | MYLC2A, MYL7/MLC2a, MYL2A, MLC-2a, MLC2a |
| Calculated Molecular Weight | 175 aa, 19 kDa |
| Observed Molecular Weight | 19 kDa |
| GenBank Accession Number | BC027915 |
| Gene Symbol | MYL7 |
| Gene ID (NCBI) | 58498 |
| RRID | AB_3673731 |
| Conjugate | CoraLite® Plus 750 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 755 nm / 780 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q01449 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MYL7, also known as myosin light chain 2a (MLC2a), is essential for heart development. The expression of MYL7 in heart is predominatantly restricted to the atrium. So its expression has been considered as useful marker for atrial cardiomyocytes.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 750 MYL7 antibody CL750-81570 | Download protocol |
| FC protocol for CL Plus 750 MYL7 antibody CL750-81570 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |