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验证数据展示

  • WB analysis using CL750-81570

    WB analysis using CL750-81570

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-81570 (MYL7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • FC experiment of C2C12 using CL750-81570

    FC experiment of C2C12 using CL750-81570

    1x10^6 C2C12 cells were intracellularly stained with 0.4 ug CoraLite® Plus 750-conjugated MYL7 Recombinant antibody (CL750-81570, Clone:2C5)(red), or 0.4 ug Isotype Control (CL750-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 750-conjugated MYL7 Recombinant antibody

MYL7 Uni-rAbTM Recombinant Antibody for WB, FC (Intra)
Cat No. CL750-81570

产品说明书

CloneNo. 2C5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, FC (Intra)

别名

MYLC2A, MYL7/MLC2a, MYL2A, MLC-2a, MLC2a

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 750
规格: 

-/ -

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经过测试的应用

Positive WB detected inrat heart tissue, mouse heart tissue
Positive FC (Intra) detected inC2C12 cells
Planning an IHC experiment? We recommend our IHCeasy MYL7 Ready-To-Use IHC Kit. MYL7 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 81570-1-PBS

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-81570 targets MYL7 in WB, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 MYL7 fusion protein Ag11058 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 myosin, light chain 7, regulatory
别名 MYLC2A, MYL7/MLC2a, MYL2A, MLC-2a, MLC2a
计算分子量 175 aa, 19 kDa
观测分子量19 kDa
GenBank蛋白编号BC027915
基因名称 MYL7
Gene ID (NCBI) 58498
RRIDAB_3673731
偶联类型 CoraLite® Plus 750 Fluorescent Dye
最大激发/发射波长755 nm / 780 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ01449
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYL7, also known as myosin light chain 2a (MLC2a), is essential for heart development. The expression of MYL7 in heart is predominatantly restricted to the atrium. So its expression has been considered as useful marker for atrial cardiomyocytes.

实验方案

Product Specific Protocols
WB protocol for CL Plus 750 MYL7 antibody CL750-81570Download protocol
FC protocol for CL Plus 750 MYL7 antibody CL750-81570Download protocol
Standard Protocols
Click here to view our Standard Protocols