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CoraLite® Plus 750-conjugated MYL7 Recombinant antibody

MYL7 Uni-rAbTM Recombinant Antibody for WB, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra)

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

2C5

Cat no : CL750-81570

Print datasheet

Synonyms

MYLC2A, MYL7/MLC2a, MYL2A, MLC-2a, MLC2a

验证数据展示

  • WB analysis using CL750-81570

    WB analysis using CL750-81570

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-81570 (MYL7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • FC experiment of C2C12 using CL750-81570

    FC experiment of C2C12 using CL750-81570

    1x10^6 C2C12 cells were intracellularly stained with 0.4 ug CoraLite® Plus 750-conjugated MYL7 Recombinant antibody (CL750-81570, Clone:2C5)(red), or 0.4 ug Isotype Control (CL750-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inrat heart tissue, mouse heart tissue
Positive FC (Intra) detected inC2C12 cells
Planning an IHC experiment? We recommend our IHCeasy MYL7 Ready-To-Use IHC Kit. MYL7 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 81570-1-PBS

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-81570 targets MYL7 in WB, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen MYL7 fusion protein Ag11058 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name myosin, light chain 7, regulatory
Synonyms MYLC2A, MYL7/MLC2a, MYL2A, MLC-2a, MLC2a
Calculated Molecular Weight 175 aa, 19 kDa
Observed Molecular Weight19 kDa
GenBank Accession NumberBC027915
Gene Symbol MYL7
Gene ID (NCBI) 58498
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission Maxima Wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ01449
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYL7, also known as myosin light chain 2a (MLC2a), is essential for heart development. The expression of MYL7 in heart is predominatantly restricted to the atrium. So its expression has been considered as useful marker for atrial cardiomyocytes.

实验方案

Product Specific Protocols
WB protocol for CL Plus 750 MYL7 antibody CL750-81570Download protocol
FC protocol for CL Plus 750 MYL7 antibody CL750-81570Download protocol
Standard Protocols
Click here to view our Standard Protocols