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验证数据展示

  • IF Staining of human rectal cancer using CL488-21642

    IF Staining of human rectal cancer using CL488-21642

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human rectal cancer tissue using CoraLite® Plus 488 MYLK antibody (CL488-21642) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CoraLite® Plus 488-conjugated MYLK Polyclonal antibody

MYLK Polyclonal Antibody for IF-P
Cat No. CL488-21642

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF-P

别名

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inhuman rectal cancer tissue
Planning an IHC experiment? We recommend our IHCeasy MYLK Ready-To-Use IHC Kit. MYLK primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 21642-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-21642 targets MYLK in IF-P applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF-P Application Description
经测试反应性 human, mouse, rat
免疫原 MYLK fusion protein Ag16306 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 myosin light chain kinase
别名
计算分子量 1914 aa, 211 kDa
观测分子量 135 kDa
GenBank蛋白编号BC113456
基因名称 MYLK
Gene ID (NCBI) 4638
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15746
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYLK(Myosin light chain kinase) is also named as MLCK, MLCK1, MYLK1 and belongs to the protein kinase superfamily. It is a key enzyme in muscle contraction. Its activation is sufficient to enhance paracellular permeability and is required for tight junction barrier regulation in response to Na+-nutrient cotransport, inflammatory cytokines, or pathogenic bacteria(PMID:20404178). The expression of smooth muscle MYLK is tissue-specific. Smooth muscle MYLK generates three different proteins, long MYLK, short MYLK, and telokin. The 130-kDa smooth muscle, or short, MYLK (130 kDa) is expressed in smooth muscles. It has 8 isoforms produced by alternative splicing and alternative initiation and our anti-MYLK antibody can recognize these isforms.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 MYLK antibody CL488-21642Download protocol
Standard Protocols
Click here to view our Standard Protocols