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验证数据展示

  • WB analysis of Jurkat using 25772-1-AP (same clone as 25772-1-PBS)

    WB analysis of Jurkat using 25772-1-AP (same clone as 25772-1-PBS)

    WB result of MYO1G antibody (25772-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MYO1G transfected Jurkat cells. This data was developed using the same antibody clone with 25772-1-PBS in a different storage buffer formulation.

  • WB analysis using 25772-1-AP (same clone as 25772-1-PBS)

    WB analysis using 25772-1-AP (same clone as 25772-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25772-1-AP (MYO1G antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25772-1-PBS in a different storage buffer formulation.

  • IF Staining of Jurkat using 25772-1-AP (same clone as 25772-1-PBS)

    IF Staining of Jurkat using 25772-1-AP (same clone as 25772-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using MYO1G antibody (25772-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25772-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

MYO1G Polyclonal antibody, PBS Only

MYO1G Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 25772-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

HA 2, Minor histocompatibility antigen HA-2, myosin IG, Unconventional myosin-Ig

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy MYO1G Ready-To-Use IHC Kit. MYO1G primary antibody included.

产品信息

25772-1-PBS targets MYO1G in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag22696

Product name: Recombinant human MYO1G protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 190-391 aa of BC113544

Sequence: RVLKQHVGERNFHAFYQLLRGSEDKQLHELHLERNPAVYNFTHQGAGLNMTVHSALDSDEQSHQAVTEAMRVIGFSPEEVESVHRILAAILHLGNIEFVETEEGGLQKEGLAVAEEALVDHVAELTATPRDLVLRSLLARTVASGGRELIEKGHTAAEASYARDACAKAVYQRLFEWVVNRINSVMEPRGRDPRRDGKDTVI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 myosin IG
别名 HA 2, Minor histocompatibility antigen HA-2, myosin IG, Unconventional myosin-Ig
计算分子量 1018 aa, 116 kDa
观测分子量110-120 kDa
GenBank蛋白编号BC113544
基因名称 MYO1G
Gene ID (NCBI) 64005
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDB0I1T2
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MYO1G is a plasma membrane-associated class I myosin that is abundant in T and B lymphocytes and mast cells and expressed specifically in the haematopoietic system. Class I myosins have been implicated in various cellular processes relying on actin-dependent membrane dynamics such as endocytosis, secretion, adhesion, motility and regulation of mechanosensitive channels (PMID: 19968988). MYO1G localizes to the plasma membrane linked to PIP2 and PIP3, is particularly enriched at cell-surface microvilli, and associates in an ATP-releasable manner to the actin cytoskeleton (PMID: 24310084). MYO1G can be detected as about 110-120 kDa.

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