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MYO6 Recombinant antibody, PBS Only (Capture/Detector)

MYO6 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241251G11

Cat no : 84085-3-PBS

Print datasheet

Synonyms

Unconventional myosin 6, myosin VI, KIAA0389, DFNB37, DFNA22

验证数据展示

  • WB analysis using 84085-3-RR (same clone as 84085-3-PBS)

    WB analysis using 84085-3-RR (same clone as 84085-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84085-3-RR (MYO6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84085-3-PBS in a different storage buffer formulation.

  • WB analysis of LNCaP using 84085-3-RR (same clone as 84085-3-PBS)

    WB analysis of LNCaP using 84085-3-RR (same clone as 84085-3-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 84085-3-RR (MYO6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84085-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01001-1

    Cytometric bead array standard curve of MP01001-1

    Cytometric bead array standard curve of MP01001-1, MYO6 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84085-3-PBS. Detection antibody: 84085-1-PBS. Standard: Ag24906. Range: 0.781-100 ng/ml.

  • Cytometric bead array standard curve of MP01001-3

    Cytometric bead array standard curve of MP01001-3

    Cytometric bead array standard curve of MP01001-3, MYO6 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84085-2-PBS. Detection antibody: 84085-3-PBS. Standard: Ag24906. Range: 0.781-100 ng/ml.

Planning an IHC experiment? We recommend our IHCeasy MYO6 Ready-To-Use IHC Kit. MYO6 primary antibody included.

产品信息

84085-3-PBS targets MYO6 as part of a matched antibody pair:

MP01001-1: 84085-3-PBS capture and 84085-1-PBS detection (validated in Cytometric bead array)

MP01001-3: 84085-2-PBS capture and 84085-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen MYO6 fusion protein Ag24906 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name myosin VI
Synonyms Unconventional myosin 6, myosin VI, KIAA0389, DFNB37, DFNA22
Calculated Molecular Weight 1285 aa, 149 kDa
Observed Molecular Weight 145-150 kDa
GenBank Accession NumberBC146764
Gene Symbol MYO6
Gene ID (NCBI) 4646
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UM54
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MYO6, an actin-based motor protein, is the only myosin known to move toward the minus end of actin filaments. MYO6 is highly expressed in the inner and outer hair cells of the ear, retina, and polarized epithelial cells such as kidney proximal tubule cells and intestinal enterocytes. And it participates in a wide range of biological processes within cells, including clathrin-mediated endocytosis, vesicular membrane traffic, polarized secretion, and autophagy (PMID: 23620821; PMID: 28591580). Previous studies showed that MYO6 is upregulated in various types of cancer, and it has been widely reported to contribute to tumor cell migration and metastasis. Some articles indicate that MYO6 is associated with prostate cancer, lung cancer, human colorectal cancer and gastric cancer (PMID: 29022908).