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验证数据展示

  • WB analysis using 18943-1-AP (same clone as 18943-1-PBS)

    WB analysis using 18943-1-AP (same clone as 18943-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 18943-1-AP (MYOD1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18943-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse embryo using 18943-1-AP (same clone as 18943-1-PBS)

    IHC staining of mouse embryo using 18943-1-AP (same clone as 18943-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 18943-1-AP (MYOD1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18943-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 18943-1-AP (same clone as 18943-1-PBS)

    IF Staining of HeLa using 18943-1-AP (same clone as 18943-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MYOD1 antibody (18943-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 18943-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

MYOD1 Polyclonal antibody, PBS Only

MYOD1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 18943-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

MYO D1, Myf-3, MYF3, Myf 3, Class C basic helix-loop-helix protein 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

18943-1-PBS targets MYOD1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MYOD1 fusion protein Ag13512 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 myogenic differentiation 1
别名 MYO D1, Myf-3, MYF3, Myf 3, Class C basic helix-loop-helix protein 1
计算分子量 320 aa, 35 kDa
观测分子量 35-45 kDa
GenBank蛋白编号BC064493
基因名称 MYOD1
Gene ID (NCBI) 4654
RRIDAB_10603467
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP15172
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MYOD1, also named as BHLHC1 or MYF3, is a 320 amino acid protein, which promotes the transcriptional activity of MYOD1 through its CDK9-mediated phosphorylation. This phosphorylation promotes its function in muscle differentiation. MYOD1 acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. MYOD1 together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. he calcualted molecular weight of MYOD1 is 34 kDa, but modified MYOD1 is about 45 kDa. (PMID: 12037670)