验证数据展示
经过测试的应用
Positive WB detected in | mouse heart tissue, human heart tissue, mouse skeletal muscle tissue, rat heart tissue |
Positive IHC detected in | mouse embryo tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
18943-1-AP targets MYOD1 in WB, IHC, IF/ICC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
文献引用应用 | WB, IHC, IF, ChIP |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse, rat, pig, rabbit, chicken, zebrafish, sheep, goat, duck |
免疫原 | MYOD1 fusion protein Ag13512 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | myogenic differentiation 1 |
别名 | MYO D1, Myf-3, MYF3, Myf 3, Class C basic helix-loop-helix protein 1 |
计算分子量 | 320 aa, 35 kDa |
观测分子量 | 35-45 kDa |
GenBank蛋白编号 | BC064493 |
基因名称 | MYOD1 |
Gene ID (NCBI) | 4654 |
RRID | AB_10603467 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P15172 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MYOD1, also named as BHLHC1 or MYF3, is a 320 amino acid protein, which promotes the transcriptional activity of MYOD1 through its CDK9-mediated phosphorylation. This phosphorylation promotes its function in muscle differentiation. MYOD1 acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. MYOD1 together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. he calcualted molecular weight of MYOD1 is 34 kDa, but modified MYOD1 is about 45 kDa. (PMID: 12037670)
实验方案
Product Specific Protocols | |
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WB protocol for MYOD1 antibody 18943-1-AP | Download protocol |
IHC protocol for MYOD1 antibody 18943-1-AP | Download protocol |
IF protocol for MYOD1 antibody 18943-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Sci Adv Bioactive fiber-reinforced hydrogel to tailor cell microenvironment for structural and functional regeneration of myotendinous junction | ||
Nat Commun The transcription factor NF-Y participates to stem cell fate decision and regeneration in adult skeletal muscle. | ||
J Cachexia Sarcopenia Muscle circRNAome profiling reveals circFgfr2 regulates myogenesis and muscle regeneration via a feedback loop. |