验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
For other applications, we recommend the unconjugated version of this antibody, 82917-7-PBS
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-82917-7 targets MYOF in IF/ICC applications and shows reactivity with human samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | human |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | myoferlin |
| Synonyms | Myoferlin, KIAA1207, Fer-1-like protein 3, FER1L3, Fer 1 like protein 3 |
| Calculated Molecular Weight | 235 kDa |
| GenBank Accession Number | NM_133337 |
| Gene Symbol | MYOF |
| Gene ID (NCBI) | 26509 |
| RRID | AB_3673116 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9NZM1 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MYOF, also named as FER1L3 and KIAA1207, belongs to the Ferlin family. MYOF is a calcium/phospholipid-binding protein that plays a role in the plasmalemma repair mechanism of endothelial cells that permits rapid resealing of membranes disrupted by mechanical stress. It is involved in endocytic recycling. MYOF is implicated in VEGF signal transduction by regulating the levels of the receptor KDR.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 MYOF antibody CL488-82917-7 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |