验证数据展示
产品信息
66506-1-PBS targets MYPT1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, rat, mouse samples.
Tested Applications | WB, IF/ICC, Indirect ELISA Application Description |
Tested Reactivity | Human, rat, mouse |
Immunogen | MYPT1 fusion protein Ag17496 种属同源性预测 |
Host / Isotype | Mouse / IgG3 |
Class | Monoclonal |
Type | Antibody |
Full Name | protein phosphatase 1, regulatory (inhibitor) subunit 12A |
Synonyms | MBS, MYPT1, PPP1R12A |
Calculated Molecular Weight | 1030 aa, 115 kDa |
Observed Molecular Weight | 115 kDa |
GenBank Accession Number | BC111752 |
Gene Symbol | MYPT1 |
Gene ID (NCBI) | 4659 |
RRID | AB_2881869 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | O14974 |
Storage Buffer | PBS Only |
Storage Conditions | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
Myosin phosphatase target subunit 1(MYPT1), which is also called PPP1R12A, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Phosphorylation of MYPT1 at Thr696 and Thr853 results in phosphatase inhibition and cytoskeletal reorganization.