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MYPT1 Monoclonal antibody, PBS Only

MYPT1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG3

Reactivity

Human, rat, mouse

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A1A9

Cat No : 66506-1-PBS

Print datasheet

Synonyms

MBS, MYPT1, PPP1R12A

验证数据展示

  • WB analysis of Raji using 66506-1-Ig (same clone as 66506-1-PBS)

    WB analysis of Raji using 66506-1-Ig (same clone as 66506-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 66506-1-Ig (MYPT1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66506-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66506-1-Ig (same clone as 66506-1-PBS)

    IF Staining of HeLa using 66506-1-Ig (same clone as 66506-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using MYPT1 antibody (66506-1-Ig, Clone: 2A1A9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66506-1-PBS in a different storage buffer formulation.

产品信息

66506-1-PBS targets MYPT1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, rat, mouse
Immunogen MYPT1 fusion protein Ag17496 种属同源性预测
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Full Name protein phosphatase 1, regulatory (inhibitor) subunit 12A
Synonyms MBS, MYPT1, PPP1R12A
Calculated Molecular Weight 1030 aa, 115 kDa
Observed Molecular Weight 115 kDa
GenBank Accession NumberBC111752
Gene Symbol MYPT1
Gene ID (NCBI) 4659
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO14974
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Myosin phosphatase target subunit 1(MYPT1), which is also called PPP1R12A, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Phosphorylation of MYPT1 at Thr696 and Thr853 results in phosphatase inhibition and cytoskeletal reorganization.