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验证数据展示

  • WB analysis of Raji using 66506-1-Ig (same clone as 66506-1-PBS)

    WB analysis of Raji using 66506-1-Ig (same clone as 66506-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 66506-1-Ig (MYPT1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66506-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66506-1-Ig (same clone as 66506-1-PBS)

    IF Staining of HeLa using 66506-1-Ig (same clone as 66506-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using MYPT1 antibody (66506-1-Ig, Clone: 2A1A9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66506-1-PBS in a different storage buffer formulation.

MYPT1 Monoclonal antibody, PBS Only

MYPT1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 66506-1-PBS

产品说明书

CloneNo. 2A1A9

宿主/亚型

Mouse / IgG3

种属反应性

Human, rat, mouse

应用

WB, IF/ICC, Indirect ELISA

别名

MBS, MYPT1, PPP1R12A

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66506-1-PBS targets MYPT1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, rat, mouse samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, rat, mouse
免疫原 MYPT1 fusion protein Ag17496 种属同源性预测
宿主/亚型 Mouse / IgG3
抗体类别 Monoclonal
产品类型 Antibody
全称 protein phosphatase 1, regulatory (inhibitor) subunit 12A
别名 MBS, MYPT1, PPP1R12A
计算分子量 1030 aa, 115 kDa
观测分子量 115 kDa
GenBank蛋白编号BC111752
基因名称 MYPT1
Gene ID (NCBI) 4659
RRIDAB_2881869
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO14974
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Myosin phosphatase target subunit 1(MYPT1), which is also called PPP1R12A, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Phosphorylation of MYPT1 at Thr696 and Thr853 results in phosphatase inhibition and cytoskeletal reorganization.