MYPT1 Monoclonal antibody

MYPT1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 66506-1-Ig

产品说明书

CloneNo. 2A1A9

宿主/亚型

Mouse / IgG3

种属反应性

Human, rat, mouse and More (1)

应用

WB, IF/ICC, ELISA

MBS, MYPT1, PPP1R12A

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inRaji cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

66506-1-Ig targets MYPT1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, rat, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 Human, rat, mouse
文献引用反应性human, rat
免疫原 MYPT1 fusion protein Ag17496 种属同源性预测
宿主/亚型 Mouse / IgG3
抗体类别 Monoclonal
产品类型 Antibody
全称 protein phosphatase 1, regulatory (inhibitor) subunit 12A
别名 MBS, MYPT1, PPP1R12A
计算分子量 1030 aa, 115 kDa
观测分子量 115 kDa
GenBank蛋白编号BC111752
基因名称 MYPT1
Gene ID (NCBI) 4659
RRIDAB_2881869
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO14974
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Myosin phosphatase target subunit 1(MYPT1), which is also called PPP1R12A, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Phosphorylation of MYPT1 at Thr696 and Thr853 results in phosphatase inhibition and cytoskeletal reorganization.

实验方案

Product Specific Protocols
WB protocol for MYPT1 antibody 66506-1-IgDownload protocol
IF protocol for MYPT1 antibody 66506-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Brain Res

The ROCK inhibitor Y-27632 ameliorates blood-spinal cord barrier disruption by reducing tight junction protein degradation via the MYPT1-MLC2 pathway after spinal cord injury in rats.

Authors - Sheng Chang
humanWB

J Virol

Hepatitis E virus ORF3 protein hijacking thioredoxin domain-containing protein 5 (TXNDC5) for its stability to promote viral particle release

Authors - Yamin Sheng
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