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CoraLite®594-conjugated MYPT1 Monoclonal antibody

MYPT1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG3

Reactivity

Human, rat, mouse

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2A1A9

Cat no : CL594-66506

Print datasheet

Synonyms

MBS, MYPT1, PPP1R12A

验证数据展示

  • IF Staining of HeLa using CL594-66506

    IF Staining of HeLa using CL594-66506

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 MYPT1 antibody (CL594-66506, Clone: 2A1A9 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66506-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66506 targets MYPT1 in IF/ICC applications and shows reactivity with Human, rat, mouse samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, rat, mouse
Immunogen MYPT1 fusion protein Ag17496 种属同源性预测
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Full Name protein phosphatase 1, regulatory (inhibitor) subunit 12A
Synonyms MBS, MYPT1, PPP1R12A
Calculated Molecular Weight 1030 aa, 115 kDa
Observed Molecular Weight 115 kDa
GenBank Accession NumberBC111752
Gene Symbol MYPT1
Gene ID (NCBI) 4659
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO14974
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Myosin phosphatase target subunit 1(MYPT1), which is also called PPP1R12A, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Phosphorylation of MYPT1 at Thr696 and Thr853 results in phosphatase inhibition and cytoskeletal reorganization.

实验方案

Product Specific Protocols
IF protocol for CL594 MYPT1 antibody CL594-66506Download protocol
Standard Protocols
Click here to view our Standard Protocols