CoraLite®594-conjugated MYPT1 Monoclonal antibody

MYPT1 Monoclonal Antibody for IF/ICC
Cat No. CL594-66506

产品说明书

CloneNo. 2A1A9

宿主/亚型

Mouse / IgG3

种属反应性

Human, rat, mouse

应用

IF/ICC

MBS, MYPT1, PPP1R12A

缓冲液配方:  PBS and Azide
PBS and Azide
规格: 

-/ -


经过测试的应用

Positive IF/ICC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66506-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66506 targets MYPT1 in IF/ICC applications and shows reactivity with Human, rat, mouse samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human, rat, mouse
免疫原 MYPT1 fusion protein Ag17496 种属同源性预测
宿主/亚型 Mouse / IgG3
抗体类别 Monoclonal
产品类型 Antibody
全称 protein phosphatase 1, regulatory (inhibitor) subunit 12A
别名 MBS, MYPT1, PPP1R12A
计算分子量 1030 aa, 115 kDa
观测分子量 115 kDa
GenBank蛋白编号BC111752
基因名称 MYPT1
Gene ID (NCBI) 4659
RRIDAB_2934729
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO14974
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Myosin phosphatase target subunit 1(MYPT1), which is also called PPP1R12A, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Phosphorylation of MYPT1 at Thr696 and Thr853 results in phosphatase inhibition and cytoskeletal reorganization.

实验方案

Product Specific Protocols
IF protocol for CL594 MYPT1 antibody CL594-66506Download protocol
Standard Protocols
Click here to view our Standard Protocols
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