验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-66506 targets MYPT1 in IF/ICC applications and shows reactivity with Human, rat, mouse samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | Human, rat, mouse |
| Immunogen | MYPT1 fusion protein Ag17496 种属同源性预测 |
| Host / Isotype | Mouse / IgG3 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | protein phosphatase 1, regulatory (inhibitor) subunit 12A |
| Synonyms | MBS, MYPT1, PPP1R12A |
| Calculated Molecular Weight | 1030 aa, 115 kDa |
| Observed Molecular Weight | 115 kDa |
| GenBank Accession Number | BC111752 |
| Gene Symbol | MYPT1 |
| Gene ID (NCBI) | 4659 |
| RRID | AB_2934729 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O14974 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Myosin phosphatase target subunit 1(MYPT1), which is also called PPP1R12A, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Phosphorylation of MYPT1 at Thr696 and Thr853 results in phosphatase inhibition and cytoskeletal reorganization.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 MYPT1 antibody CL594-66506 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |