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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated MCP-1/CCL2 Monoclonal antibody

MCP-1/CCL2 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG1

Reactivity

mouse

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B9F7

Cat no : CL488-66272

Print datasheet

Synonyms

Ccl2, Mcp1, MCP-1, chemokine (C C motif) ligand 2, CCL-2

验证数据展示

  • IF Staining of mouse liver using CL488-66272

    IF Staining of mouse liver using CL488-66272

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CoraLite® Plus 488 Mcp1 antibody (CL488-66272, Clone: 1B9F7 ) at dilution of 1:200.

经过测试的应用

Positive IF-P detected inmouse liver tissue

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66272 targets MCP-1/CCL2 in IF-P applications and shows reactivity with mouse samples.

Tested Applications IF-P Application Description
Tested Reactivity mouse
Immunogen MCP-1/CCL2 fusion protein Ag24085 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name chemokine (C-C motif) ligand 2
Synonyms Ccl2, Mcp1, MCP-1, chemokine (C C motif) ligand 2, CCL-2
Observed Molecular Weight 26 kDa
GenBank Accession NumberNM-011333
Gene Symbol MCP-1/CCL2
Gene ID (NCBI) 20296
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Monocyte chemoattractant protein-1 (MCP-1/CCL2) is one of the key chemokines that regulate migration and infiltration of monocytes/macrophages. Both CCL2 and its receptor CCR2 have been demonstrated to be induced and involved in various diseases. Migration of monocytes from the blood stream across the vascular endothelium is required for routine immunological surveillance of tissues, as well as in response to inflammation.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 MCP-1/CCL2 antibody CL488-66272Download protocol
Standard Protocols
Click here to view our Standard Protocols