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Melanophilin Monoclonal antibody, PBS Only

Melanophilin Monoclonal Antibody for WB,IHC,IF,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB,IHC,IF,ELISA

Conjugate

Unconjugated

CloneNo.

1A8D12

Cat No : 66092-1-PBS

Print datasheet

Synonyms

Exophilin 3, melanophilin, MLPH, SLAC2 A, SLAC2A, Synaptotagmin like protein 2a

验证数据展示

  • WB analysis using 66092-1-Ig (same clone as 66092-1-PBS)

    WB analysis using 66092-1-Ig (same clone as 66092-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66092-1-Ig (Melanophilin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66092-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 66092-1-Ig (same clone as 66092-1-PBS)

    IHC staining of human kidney using 66092-1-Ig (same clone as 66092-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66092-1-Ig (Melanophilin antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66092-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 66092-1-Ig (same clone as 66092-1-PBS)

    IHC staining of human kidney using 66092-1-Ig (same clone as 66092-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66092-1-Ig (Melanophilin antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66092-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66092-1-Ig (same clone as 66092-1-PBS)

    IF Staining of HepG2 using 66092-1-Ig (same clone as 66092-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66092-1-Ig (Melanophilin antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66092-1-PBS in a different storage buffer formulation.

产品信息

66092-1-PBS targets Melanophilin in WB, IF, IHC, ELISA applications and shows reactivity with human, pig samples.

Tested Applications WB,IHC,IF,ELISA Application Description
Tested Reactivity human, pig
Immunogen Melanophilin fusion protein Ag19286 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name melanophilin
Synonyms Exophilin 3, melanophilin, MLPH, SLAC2 A, SLAC2A, Synaptotagmin like protein 2a
Calculated Molecular Weight 66 kDa
Observed Molecular Weight 66 kDa
GenBank Accession NumberBC001653
Gene Symbol MLPH
Gene ID (NCBI) 79083
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9BV36
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

背景介绍

Melanophilin (MLPH) is a member of the exophilin subfamily of Rab effector proteins. MLPH is a modular protein, forming a ternary complex with the small Ras-related GTPase Rab27A in its GTP-bound form and the motor protein myosin Va. It regulates retention of melanosomes at the peripheral actin cytoskeleton of melanocytes, a process essential for normal mammalian pigmentation. Defects in MLPH are a cause of Griscelli syndrome type 3 (GS3), which is characterized by a silver-gray hair color and abnormal pigment distribution in the hair shaft.