MLPH Monoclonal antibody

MLPH Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66092-1-Ig

产品说明书

CloneNo. 1A8D12

宿主/亚型

Mouse / IgG1

种属反应性

human, pig and More (1)

应用

WB, IHC, IF/ICC, ELISA and More (1)

SlaC2-a, SLAC2A, SLAC2 A, Melanophilin, Exophilin-3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells, pig brain tissue, PC-3 cells, Jurkat cells, human brain tissue
Positive IHC detected inhuman kidney tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66092-1-Ig targets MLPH in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, pig samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, pig
文献引用反应性human, rat
免疫原 MLPH fusion protein Ag19286 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 melanophilin
别名 SlaC2-a, SLAC2A, SLAC2 A, Melanophilin, Exophilin-3
计算分子量 66 kDa
观测分子量 66 kDa
GenBank蛋白编号BC001653
基因名称 MLPH
Gene ID (NCBI) 79083
RRIDAB_11232039
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9BV36
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Melanophilin (MLPH) is a member of the exophilin subfamily of Rab effector proteins. MLPH is a modular protein, forming a ternary complex with the small Ras-related GTPase Rab27A in its GTP-bound form and the motor protein myosin Va. It regulates retention of melanosomes at the peripheral actin cytoskeleton of melanocytes, a process essential for normal mammalian pigmentation. Defects in MLPH are a cause of Griscelli syndrome type 3 (GS3), which is characterized by a silver-gray hair color and abnormal pigment distribution in the hair shaft.

实验方案

Product Specific Protocols
WB protocol for MLPH antibody 66092-1-IgDownload protocol
IHC protocol for MLPH antibody 66092-1-IgDownload protocol
IF protocol for MLPH antibody 66092-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB,IF

Int J Biol Macromol

A multifunctional polydopamine/genipin/alendronate nanoparticle licences fibrin hydrogels osteoinductive and immunomodulatory potencies for repairing bone defects

Authors - Wentao Shi
humanWB

Mol Med Rep

Knockdown of microRNA‑143‑5p by STTM technology affects eumelanin and pheomelanin production in melanocytes.

Authors - Shuhui Qi
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