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验证数据展示

  • WB analysis using 80219-1-RR

    WB analysis using 80219-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80219-1-RR (Mono/Di-Methyl-Histone H3 (Lys9) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 80219-1-RR

    IF Staining of HeLa using 80219-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Mono/Di-Methyl-Histone H3 (Lys9) antibody (80219-1-RR, Clone: 1E12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HeLa using 80219-1-RR

    IF Staining of HeLa using 80219-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Mono/Di-Methyl-Histone H3 (Lys9) antibody (80219-1-RR, Clone: 1E12 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Mono/Di-Methyl-Histone H3 (Lys9) Recombinant antibody

Mono/Di-Methyl-Histone H3 (Lys9) Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 80219-1-RR
CloneNo. 1E12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

HIST1H3A, 1E12, Histone-H3, HistoneH3, Histone H3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inTHP-1 cells, K-562 cells, Jurkat cells, HeLa cells, HEK-293 cells, NIH/3T3 cells, C2C12 cells, HSC-T6 cells, PC-12 cells
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy Phospho-Histone H3 (Ser10) Ready-To-Use IHC Kit. Phospho-Histone H3 (Ser10) primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:5000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80219-1-RR targets Mono/Di-Methyl-Histone H3 (Lys9) in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name histone cluster 1, H3a
Synonyms HIST1H3A, 1E12, Histone-H3, HistoneH3, Histone H3
Observed Molecular Weight15-18 kDa
GenBank Accession NumberBC066245
Gene Symbol HIST1H3A
Gene ID (NCBI) 8350
RRIDAB_2918878
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP68431
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Histones, including H1/H5 (linker histones), H2, H3, and H4 (core histones), are nucleic proteins which interact with DNA to form the nucleosomes and play important roles in gene regulation and DNA replication. Histone proteins are highly post-translationally modified while Histone H3 is the most extensively modified. Methylation of Histone H3 at lysine 9 is linked to transcriptional repression. This antibody is specific to monomethyl or dimethyl-Histone H3 while it does not recognize trimethyl-Histone H3 (Lys9). It is also named as H3K9me1/2.

实验方案

Product Specific Protocols
WB protocol for Mono/Di-Methyl-Histone H3 (Lys9) antibody 80219-1-RRDownload protocol
IF protocol for Mono/Di-Methyl-Histone H3 (Lys9) antibody 80219-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols