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Mono-Methyl-Histone H3 (Lys9) Recombinant antibody

Mono-Methyl-Histone H3 (Lys9) Uni-rAbTM Recombinant Antibody for WB, Dot Blot, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, Dot Blot, ELISA

Conjugate

Unconjugated

CloneNo.

3C23

Cat no : 82821-6-RR

Print datasheet

Synonyms

HIST1H3A, 3C23, Histone-H3, HistoneH3, Histone H3

验证数据展示

  • Dot Blot experiment of peptide using 82821-6-RR

    Dot Blot experiment of peptide using 82821-6-RR

    Dot blot analysis was used to confirm the specificity of 82821-6-RR Mono-Methyl-Histone H3 (Lys9) antibody. Peptides were spotted onto NC and probed with antibody at 1 µg/ml. The amount of peptide (ug/mL) spotted is indicated next to each row.

  • WB analysis using 82821-6-RR

    WB analysis using 82821-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82821-6-RR (Mono-Methyl-Histone H3 (Lys9) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, K-562 cells, THP-1 cells, HSC-T6 cells
Positive Dot Blot detected inpeptide
Planning an IHC experiment? We recommend our IHCeasy Phospho-Histone H3 (Ser10) Ready-To-Use IHC Kit. Phospho-Histone H3 (Ser10) primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
DOT BLOTDOT BLOT : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82821-6-RR targets Mono-Methyl-Histone H3 (Lys9) in WB, Dot Blot, ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, Dot Blot, ELISA Application Description
Tested Reactivity human, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name histone cluster 1, H3a
Synonyms HIST1H3A, 3C23, Histone-H3, HistoneH3, Histone H3
Observed Molecular Weight15 kDa
GenBank Accession NumberBC066245
Gene Symbol HIST1H3A
Gene ID (NCBI) 8350
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP68431
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Histones, including H1/H5 (linker histones), H2, H3, and H4 (core histones), are nucleic proteins which interact with DNA to form the nucleosomes and play important roles in gene regulation and DNA replication. Histone proteins are highly post-translationally modified while Histone H3 is the most extensively modified. Methylation of Histone H3 at lysine 9 is linked to transcriptional repression.

实验方案

Product Specific Protocols
WB protocol for Mono-Methyl-Histone H3 (Lys9) antibody 82821-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols