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验证数据展示

  • Dot Blot experiment of peptide using 82821-6-RR

    Dot Blot experiment of peptide using 82821-6-RR

    Dot blot analysis was used to confirm the specificity of 82821-6-RR Mono-Methyl-Histone H3 (Lys9) antibody. Peptides were spotted onto NC and probed with antibody at 1 µg/ml. The amount of peptide (ug/mL) spotted is indicated next to each row.

  • WB analysis using 82821-6-RR

    WB analysis using 82821-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82821-6-RR (Mono-Methyl-Histone H3 (Lys9) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Mono-Methyl-Histone H3 (Lys9) Recombinant antibody

Mono-Methyl-Histone H3 (Lys9) Uni-rAbTM Recombinant Antibody for WB, Dot Blot, ELISA
Cat No. 82821-6-RR

产品说明书

CloneNo. 3C23

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, Dot Blot, ELISA

别名

HIST1H3A, 3C23, Histone-H3, HistoneH3, Histone H3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, K-562 cells, THP-1 cells, HSC-T6 cells
Positive Dot Blot detected inpeptide
Planning an IHC experiment? We recommend our IHCeasy Phospho-Histone H3 (Ser10) Ready-To-Use IHC Kit. Phospho-Histone H3 (Ser10) primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
DOT BLOTDOT BLOT : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82821-6-RR targets Mono-Methyl-Histone H3 (Lys9) in WB, Dot Blot, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, Dot Blot, ELISA Application Description
经测试反应性 human, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 histone cluster 1, H3a
别名 HIST1H3A, 3C23, Histone-H3, HistoneH3, Histone H3
观测分子量15 kDa
GenBank蛋白编号BC066245
基因名称 HIST1H3A
Gene ID (NCBI) 8350
RRIDAB_3670563
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP68431
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Histones, including H1/H5 (linker histones), H2, H3, and H4 (core histones), are nucleic proteins which interact with DNA to form the nucleosomes and play important roles in gene regulation and DNA replication. Histone proteins are highly post-translationally modified while Histone H3 is the most extensively modified. Methylation of Histone H3 at lysine 9 is linked to transcriptional repression.

实验方案

Product Specific Protocols
WB protocol for Mono-Methyl-Histone H3 (Lys9) antibody 82821-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols