• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Mouse CD14 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: mouse

Detection: mouse

Applications

Sandwich ELISA

Range

Sandwich ELISA: 31.25-2000 pg/mL

Cat No : MP00313-4

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP00313-4, Mouse CD14 Monoclonal Matched Antibody Pair - PBS only. 83297-7-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0847. 83297-1-PBS was HRP conjugated as the detection antibody. Range: 31.25-2000 pg/mL.

    Sandwich ELISA standard curve of MP00313-4

    Sandwich ELISA standard curve of MP00313-4, Mouse CD14 Monoclonal Matched Antibody Pair - PBS only. 83297-7-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0847. 83297-1-PBS was HRP conjugated as the detection antibody. Range: 31.25-2000 pg/mL.

  • Serum of sixteen mice was measured. The CD14 concentration of detected samples was determined to be 113.29 ng/mL with a range of 83.24-160.50 ng/mL.

    Sample test of MP00313-4

    Serum of sixteen mice was measured. The CD14 concentration of detected samples was determined to be 113.29 ng/mL with a range of 83.24-160.50 ng/mL.

  • Mouse splenocytes (1 x 10^6 cells/mL) were cultured for 3 days in RPMI supplemented with 10% fetal calf serum, 100 ng/mL of recombinant mouse IFN-γ and 1 μg/mL of LPS.The mean CD-14 concentration was determined to be 2,187.4 pg/mL in unstimulated spleen, 8,939.2 pg/mL in IFN-γ and LPS stimulated spleen supernatant.

    Sample test of MP00313-4

    Mouse splenocytes (1 x 10^6 cells/mL) were cultured for 3 days in RPMI supplemented with 10% fetal calf serum, 100 ng/mL of recombinant mouse IFN-γ and 1 μg/mL of LPS.The mean CD-14 concentration was determined to be 2,187.4 pg/mL in unstimulated spleen, 8,939.2 pg/mL in IFN-γ and LPS stimulated spleen supernatant.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

MP00313-4 targets CD14 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 31.25-2000 pg/mL (Sandwich ELISA);

Capture antibody: Mouse CD14 Recombinant antibody, PBS Only (Capture) 83297-7-PBS (240225F2). 100 μg. Concentration 1 mgl/ml.

Detection antibody: Mouse CD14 Recombinant antibody, PBS Only (Detector) 83297-1-PBS (240225A8). 100 μg. Concentration 1 mgl/ml.

Alternative CD14 matched antibody pairs: MP00058-1, MP00058-2, MP00058-3, MP00058-4, MP00313-1, MP50282-1, MP50282-2

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 83297-7-PBS
Tested Reactivity mouse
Immunogen Fusion Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD14 antigen
Synonyms CD14 antigen, 240225F2
GenBank accession numberNM_009841.4
Gene symbol Cd14
UniprotP10810
Gene ID (NCBI) 12475
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
Catalog Number 83297-1-PBS
Tested Reactivity mouse
Immunogen Fusion Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD14 antigen
Synonyms CD14 antigen, 240225A8
GenBank accession numberNM_009841.4
Gene symbol Cd14
UniprotP10810
Gene ID (NCBI) 12475
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C