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Mouse NGAL Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: mouse

Detection: mouse

Applications

Sandwich ELISA

Range

Sandwich ELISA: 6.25-400 pg/mL

Cat No : MP00302-4

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP00302-4, Mouse NGAL Monoclonal Matched Antibody Pair - PBS only. 83102-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0572. 83102-5-PBS was HRP conjugated as the detection antibody. Range: 6.25-400 pg/mL.

    Sandwich ELISA standard curve of MP00302-4

    Sandwich ELISA standard curve of MP00302-4, Mouse NGAL Monoclonal Matched Antibody Pair - PBS only. 83102-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0572. 83102-5-PBS was HRP conjugated as the detection antibody. Range: 6.25-400 pg/mL.

  • Serum of sixteen mice were measured. The NGAL concentration of detected samples was determined to be 27.0 ng/mL with a range of 16.3 - 72.7 ng/mL.

    Sample test of MP00302-4

    Serum of sixteen mice were measured. The NGAL concentration of detected samples was determined to be 27.0 ng/mL with a range of 16.3 - 72.7 ng/mL.

  • Urine of eight mice were measured. The NGAL concentration of detected samples was determined to be 63.5 ng/mL with a range of 31.0 - 113.3 ng/mL.

    Sample test of MP00302-4

    Urine of eight mice were measured. The NGAL concentration of detected samples was determined to be 63.5 ng/mL with a range of 31.0 - 113.3 ng/mL.

  • Mouse reticulum cell sarcoma macrophage cells (0.5 x 10^6 cells/mL) were cultured for 1 day in DMEM supplemented with 10% fetal bovine serum containing 20 ng/mL of recombinant mouse IFN-γ and 1 μg/mL of lipopolysaccharide. The mean NGAL concentration was determined to be 2.3 ng/mL in unstimulated J774A.1 supernatant, 248.3 ng/mL in stimulated J774A.1 supernatant.

    Sample test of MP00302-4

    Mouse reticulum cell sarcoma macrophage cells (0.5 x 10^6 cells/mL) were cultured for 1 day in DMEM supplemented with 10% fetal bovine serum containing 20 ng/mL of recombinant mouse IFN-γ and 1 μg/mL of lipopolysaccharide. The mean NGAL concentration was determined to be 2.3 ng/mL in unstimulated J774A.1 supernatant, 248.3 ng/mL in stimulated J774A.1 supernatant.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

MP00302-4 targets NGAL in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 6.25-400 pg/mL (Sandwich ELISA);

Capture antibody: Mouse Lipocalin-2/Ngal Recombinant antibody, PBS Only (Capture) 83102-1-PBS (230372D6). 100 μg. Concentration 1 mgl/ml.

Detection antibody: Mouse NGAL Recombinant antibody, PBS Only (Detector) 83102-5-PBS (230372C6). 100 μg. Concentration 1 mgl/ml.

Alternative NGAL matched antibody pairs: MP00302-1, MP00302-2, MP00302-3, MP00770-1

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 83102-1-PBS
Tested Reactivity mouse
Immunogen Recombinant protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name lipocalin 2
Synonyms Lcn2, Lipocalin-2, Oncogene 24p3, Neutrophil gelatinase-associated lipocalin, Lipocalin 2
GenBank accession numberNM_008491.1
Gene symbol NGAL
UniprotP11672
Gene ID (NCBI) 16819
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
Catalog Number 83102-5-PBS
Tested Reactivity mouse
Immunogen Recombinant protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name lipocalin 2
Synonyms Lcn2, Lipocalin-2, Oncogene 24p3, Neutrophil gelatinase-associated lipocalin, Lipocalin 2
GenBank accession numberNM_008491.1
Gene symbol NGAL
UniprotP11672
Gene ID (NCBI) 16819
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C