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Mouse NGAL Recombinant antibody, PBS Only (Detector)

NGAL Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

230372C6

Cat no : 83102-5-PBS

Print datasheet

Synonyms

Lcn2, Lipocalin-2, Oncogene 24p3, Neutrophil gelatinase-associated lipocalin, Lipocalin 2

验证数据展示

  • Sandwich ELISA standard curve of MP00302-4

    Sandwich ELISA standard curve of MP00302-4

    Sandwich ELISA standard curve of MP00302-4, Mouse NGAL Monoclonal Matched Antibody Pair - PBS only. 83102-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0572. 83102-5-PBS was HRP conjugated as the detection antibody. Range: 6.25-400 pg/mL.

  • Sample test of MP00302-4

    Sample test of MP00302-4

    Urine of eight mice were measured. The NGAL concentration of detected samples was determined to be 63.5 ng/mL with a range of 31.0 - 113.3 ng/mL.

  • Sample test of MP00302-4

    Sample test of MP00302-4

    Mouse reticulum cell sarcoma macrophage cells (0.5 x 10^6 cells/mL) were cultured for 1 day in DMEM supplemented with 10% fetal bovine serum containing 20 ng/mL of recombinant mouse IFN-γ and 1 μg/mL of lipopolysaccharide. The mean NGAL concentration was determined to be 2.3 ng/mL in unstimulated J774A.1 supernatant, 248.3 ng/mL in stimulated J774A.1 supernatant.

  • Sample test of MP00302-4

    Sample test of MP00302-4

    Serum of sixteen mice were measured. The NGAL concentration of detected samples was determined to be 27.0 ng/mL with a range of 16.3 - 72.7 ng/mL.

  • Cytometric bead array standard curve of MP00302-2

    Cytometric bead array standard curve of MP00302-2

    Cytometric bead array standard curve of MP00302-2, Mouse NGAL Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83102-7-PBS. Detection antibody: 83102-5-PBS. Standard: Eg0572. Range: 0.625-20 ng/mL.

产品信息

83102-5-PBS targets NGAL as part of a matched antibody pair:

MP00302-2: 83102-7-PBS capture and 83102-5-PBS detection (validated in Cytometric bead array)

MP00302-4: 83102-1-PBS capture and 83102-5-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity mouse
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name lipocalin 2
Synonyms Lcn2, Lipocalin-2, Oncogene 24p3, Neutrophil gelatinase-associated lipocalin, Lipocalin 2
Calculated Molecular Weight23KD
GenBank Accession NumberNM_008491.1
Gene Symbol NGAL
Gene ID (NCBI) 16819
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP11672
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C