验证数据展示
经过测试的应用
| Positive IF-P detected in | human gliomas tissue |
| Positive FC (Intra) detected in | SH-SY5Y cells |
For other applications, we recommend the unconjugated version of this antibody, 12495-1-AP
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
CL594-12495 targets Mu Crystallin in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF-P, FC (Intra) Application Description |
| Cited Applications | IF |
| Tested Reactivity | human, mouse, rat |
| Immunogen | Mu Crystallin fusion protein Ag3161 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | crystallin, mu |
| Synonyms | CRYM, NADP-regulated thyroid-hormone-binding protein, Ketimine reductase mu-crystallin, EC:1.5.1.25, DFNA40 |
| Calculated Molecular Weight | 314 aa, 34 kDa |
| Observed Molecular Weight | 34 kDa |
| GenBank Accession Number | BC018061 |
| Gene Symbol | Mu Crystallin |
| Gene ID (NCBI) | 1428 |
| RRID | AB_2919784 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14894 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
mu Crystallin(thiomorpholine-carboxylate dehydrogenase) is also named as THBP, CRYM, ketimine reductase and belongs to the ornithine cyclodeaminase family. This protein catalyzes the reduction of imine bonds in brain substrates that may include cystathionine ketimine (CysK) and lanthionine ketimine (LK). It is also involved in the regulation of the free intracellular concentration of triiodothyronine and access to its nuclear receptor.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 Mu Crystallin antibody CL594-12495 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |