验证数据展示
经过测试的应用
| Positive IF-P detected in | human gliomas tissue |
| Positive FC (Intra) detected in | SH-SY5Y cells |
For other applications, we recommend the unconjugated version of this antibody, 12495-1-AP
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
CL594-12495 targets Mu Crystallin in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF-P, FC (Intra) Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | Mu Crystallin fusion protein Ag3161 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | crystallin, mu |
| 别名 | CRYM, NADP-regulated thyroid-hormone-binding protein, Ketimine reductase mu-crystallin, EC:1.5.1.25, DFNA40 |
| 计算分子量 | 314 aa, 34 kDa |
| 观测分子量 | 34 kDa |
| GenBank蛋白编号 | BC018061 |
| 基因名称 | Mu Crystallin |
| Gene ID (NCBI) | 1428 |
| RRID | AB_2919784 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q14894 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
mu Crystallin(thiomorpholine-carboxylate dehydrogenase) is also named as THBP, CRYM, ketimine reductase and belongs to the ornithine cyclodeaminase family. This protein catalyzes the reduction of imine bonds in brain substrates that may include cystathionine ketimine (CysK) and lanthionine ketimine (LK). It is also involved in the regulation of the free intracellular concentration of triiodothyronine and access to its nuclear receptor.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 Mu Crystallin antibody CL594-12495 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |