• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

MultiPro® 5CFLX Anti-Human CD35 (E11)

CD35 Monoclonal Antibody for Single Cell (Intra), Single Cell

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

Single Cell (Intra), Single Cell

Conjugate

5CFLX

Barcode Sequence

AATGAATTGGATTCG

CloneNo.

E11

Cat no : G65254-1-5C

Print datasheet

Synonyms

C3b/C4b receptor, C3BR, CD35, Complement receptor type 1, CR1, KN

验证数据展示

  • Single Cell Sequencing experiment G65254-1-5C on Resting PBMC

    Single Cell Sequencing experiment G65254-1-5C on Resting PBMC

    G65254-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G65254-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G65254-1-5C on PBMC treated with 4hr LPS + TI

    G65254-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G65254-1-5C on Resting PBMC

    Single Cell Sequencing experiment G65254-1-5C on Resting PBMC

    G65254-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

  • Single Cell Sequencing experiment G65254-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G65254-1-5C on PBMC treated with 4hr LPS + TI

    G65254-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

经过测试的应用

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.
Positive Single Cell detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.
Planning an IHC experiment? We recommend our IHCeasy CR1/CD35 Ready-To-Use IHC Kit. CR1/CD35 primary antibody included.

推荐稀释比

ApplicationDilution
SINGLE CELL (INTRA)<0.5ug/test
SINGLE CELL<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

G65254-1-5C targets CD35 in Single Cell (Intra), Single Cell applications and shows reactivity with Human samples.

Tested Applications Single Cell (Intra), Single Cell Application Description
Tested Reactivity Human
Immunogen Human CD35 from Acute monocytic leukemia cells and normal blood monocytes 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Oligo Conjugate
Type Monoclonal
Full Name MultiPro® 5CFLX Anti-Human CD35 (E11)
Synonyms C3b/C4b receptor, C3BR, CD35, Complement receptor type 1, CR1, KN
Calculated Molecular Weight 224 kDa
GenBank Accession NumberNM_000573
Gene Symbol CR1
Gene ID (NCBI) 1378
ENSEMBL Gene IDENSG00000203710
Conjugate 5CFLX
Full Oligo SequenceCGGAGATGTGTATAAGAGACAGAATGAATTGGATTCGCCCATATAAGAAA
Barcode SequenceAATGAATTGGATTCG
Form Liquid
UNIPROT IDP17927
Storage Buffer PBS with 1mM EDTA and 0.09% sodium azide
Storage Conditions2-8°C Stable for one year after shipment.

背景介绍

CD35, also known as complement receptor type 1 (CR1) or C3b/C4b receptor, is a single-chain glycoprotein consisting of 30 repeating homologous protein domains known as short consensus repeats (~60 amino acids each) followed by transmembrane and cytoplasmic domains (PMID: 8765013). CD35 is variably expressed by granulocytes, monocytes, B cells, some T cells, erythrocytes, follicular dendritic cells, Langerhans cells, and glomerular podocytes (PMID: 19004497). CD35 acts as a receptor for C3b and C4b and plays important roles in the regulation of the complement cascade and clearance of immune complexes.

Protocols

Our current MultiPro staining protocol for the cocktailDownload protocol
10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdfDownload protocol