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验证数据展示

  • IF Staining of mouse brain using CL488-10458

    IF Staining of mouse brain using CL488-10458

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 Myelin basic protein antibody (CL488-10458) at dilution of 1:200.

  • IF Staining of mouse brain using CL488-10458

    IF Staining of mouse brain using CL488-10458

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 Myelin basic protein antibody (CL488-10458) at dilution of 1:200.

CoraLite® Plus 488-conjugated MBP Polyclonal antibody

MBP Polyclonal Antibody for IF-P
Cat No. CL488-10458

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P

Synonyms

myelin basic protein

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse brain tissue
Planning an IHC experiment? We recommend our IHCeasy MBP Ready-To-Use IHC Kit. MBP primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10458-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10458 targets MBP in IF-P applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF-P Application Description
Tested Reactivity human, mouse, rat
Immunogen MBP fusion protein Ag0713 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name myelin basic protein
Synonyms myelin basic protein
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 14-23 kDa
GenBank Accession NumberBC008749
Gene Symbol Myelin basic protein
Gene ID (NCBI) 4155
RRIDAB_3083878
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP02686
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MBP belongs to the myelin basic protein family. The classic group of MBP isoforms (isoform 4-isoform 14) are the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. MBP has six isoforms. Catalog#10458-1-AP is capable of recognizing multiple isoforms of MBP.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 MBP antibody CL488-10458Download protocol
Standard Protocols
Click here to view our Standard Protocols