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Myoglobin Monoclonal antibody, PBS Only

Myoglobin Monoclonal Antibody for Indirect ELISA, IHC, WB

Host / Isotype

Mouse / IgG1

Reactivity

rabbit, pig, rat, mouse, human

Applications

Indirect ELISA, IHC, WB

Conjugate

Unconjugated

CloneNo.

1A4F7

Cat no : 66205-1-PBS

Print datasheet

Synonyms

MB, Pseudoperoxidase MB, Nitrite reductase MB, EC:1.7.-.-, EC:1.11.1.-

验证数据展示

  • WB analysis of human skeletal muscle using 66205-1-Ig (same clone as 66205-1-PBS)

    WB analysis of human skeletal muscle using 66205-1-Ig (same clone as 66205-1-PBS)

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66205-1-Ig (Myoglobin antibody at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66205-1-PBS in a different storage buffer formulation.

  • IHC staining of rat heart using 66205-1-Ig (same clone as 66205-1-PBS)

    IHC staining of rat heart using 66205-1-Ig (same clone as 66205-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66205-1-Ig (Myoglobin antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66205-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 66205-1-Ig (same clone as 66205-1-PBS)

    IHC staining of human heart using 66205-1-Ig (same clone as 66205-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66205-1-Ig (Myoglobin antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66205-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse tongue using 66205-1-Ig (same clone as 66205-1-PBS)

    IHC staining of mouse tongue using 66205-1-Ig (same clone as 66205-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse tongue tissue slide using 66205-1-Ig (Myoglobin antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66205-1-PBS in a different storage buffer formulation.

  • FC experiment of C2C12 using 66205-1-Ig (same clone as 66205-1-PBS)

    FC experiment of C2C12 using 66205-1-Ig (same clone as 66205-1-PBS)

    1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human Myoglobin (66205-1-Ig, Clone:1A4F7) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66205-1-PBS in a different storage buffer formulation.

产品信息

66205-1-PBS targets Myoglobin in Indirect ELISA, IHC, WB applications and shows reactivity with rabbit, pig, rat, mouse, human samples.

Tested Applications Indirect ELISA, IHC, WB Application Description
Tested Reactivity rabbit, pig, rat, mouse, human
Immunogen Myoglobin fusion protein Ag8979 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name myoglobin
Synonyms MB, Pseudoperoxidase MB, Nitrite reductase MB, EC:1.7.-.-, EC:1.11.1.-
Calculated Molecular Weight 154 aa, 17 kDa
Observed Molecular Weight 17-18 kDa
GenBank Accession NumberBC014547
Gene Symbol Myoglobin
Gene ID (NCBI) 4151
RRIDAB_2881596
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP02144
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Myoglobin is a cytoplasmic hemoprotein that is expressed primarily in cardiomyocytes and oxidative skeletal muscle fibers, functioning on facilitating oxygen transport and modulating nitric oxide homeostasis within cardiac and skeletal myocytes. Recent studies indicated that myoglobin was also expressed in non-muscle tissues. This antibody well recognized endogenous myoglobin in muscle lysates.