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验证数据展示

  • WB analysis of human skeletal muscle using 66205-1-Ig

    WB analysis of human skeletal muscle using 66205-1-Ig

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66205-1-Ig (Myoglobin antibody at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat heart using 66205-1-Ig

    IHC staining of rat heart using 66205-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66205-1-Ig (Myoglobin antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human heart using 66205-1-Ig

    IHC staining of human heart using 66205-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66205-1-Ig (Myoglobin antibody at dilution of 1:200 (under 10x lens).

  • IHC staining of mouse tongue using 66205-1-Ig

    IHC staining of mouse tongue using 66205-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse tongue tissue slide using 66205-1-Ig (Myoglobin antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of C2C12 using 66205-1-Ig

    FC experiment of C2C12 using 66205-1-Ig

    1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human Myoglobin (66205-1-Ig, Clone:1A4F7) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Myoglobin Monoclonal antibody

Myoglobin Monoclonal Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 66205-1-Ig

产品说明书

CloneNo. 1A4F7
引用文献(1)

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IHC, FC (Intra), ELISA

别名

MB, Pseudoperoxidase MB, Nitrite reductase MB, EC:1.7.-.-, EC:1.11.1.-

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman skeletal muscle tissue, pig heart tissue, rat heart tissue, mouse heart tissue, rabbit heart tissue, human heart tissue
Positive IHC detected inhuman heart tissue, mouse tongue tissue, rat heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inC2C12 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:80000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

66205-1-Ig targets Myoglobin in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat, pig, rabbit
文献引用反应性pig
免疫原 Myoglobin fusion protein Ag8979 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 myoglobin
别名 MB, Pseudoperoxidase MB, Nitrite reductase MB, EC:1.7.-.-, EC:1.11.1.-
计算分子量 154 aa, 17 kDa
观测分子量 17-18 kDa
GenBank蛋白编号BC014547
基因名称 Myoglobin
Gene ID (NCBI) 4151
RRIDAB_2881596
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP02144
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Myoglobin is a cytoplasmic hemoprotein that is expressed primarily in cardiomyocytes and oxidative skeletal muscle fibers, functioning on facilitating oxygen transport and modulating nitric oxide homeostasis within cardiac and skeletal myocytes. Recent studies indicated that myoglobin was also expressed in non-muscle tissues. This antibody well recognized endogenous myoglobin in muscle lysates.

实验方案

Product Specific Protocols
WB protocol for Myoglobin antibody 66205-1-IgDownload protocol
IHC protocol for Myoglobin antibody 66205-1-IgDownload protocol
FC protocol for Myoglobin antibody 66205-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
pigWB

Stress Biol

Time-restricted feeding relieves high temperature-induced impairment on meat quality by activating the Nrf2/HO-1 pathway, modification of muscle fiber composition, and enriching the polyunsaturated fatty acids in pigs

Authors - Zhaojian Li
View Article