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CoraLite® Plus 488-conjugated Myoglobin Monoclonal antibody

Myoglobin Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A4F7

Cat no : CL488-66205

Print datasheet

Synonyms

MB, Pseudoperoxidase MB, Nitrite reductase MB, EC:1.7.-.-, EC:1.11.1.-

验证数据展示

  • FC experiment of C2C12 using CL488-66205

    FC experiment of C2C12 using CL488-66205

    1X10^6 C2C12 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human Myoglobin (CL488-66205, Clone:1A4F7) (red), or 0.8 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inC2C12 cells
For other applications, we recommend the unconjugated version of this antibody, 66205-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66205 targets Myoglobin in FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen Myoglobin fusion protein Ag8979 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name myoglobin
Synonyms MB, Pseudoperoxidase MB, Nitrite reductase MB, EC:1.7.-.-, EC:1.11.1.-
Calculated Molecular Weight 154 aa, 17 kDa
Observed Molecular Weight 17-18 kDa
GenBank Accession NumberBC014547
Gene Symbol Myoglobin
Gene ID (NCBI) 4151
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP02144
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Myoglobin is a cytoplasmic hemoprotein that is expressed primarily in cardiomyocytes and oxidative skeletal muscle fibers, functioning on facilitating oxygen transport and modulating nitric oxide homeostasis within cardiac and skeletal myocytes. Recent studies indicated that myoglobin was also expressed in non-muscle tissues. This antibody well recognized endogenous myoglobin in muscle lysates.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 Myoglobin antibody CL488-66205Download protocol
Standard Protocols
Click here to view our Standard Protocols