验证数据展示
经过测试的应用
| Positive WB detected in | pig heart tissue, rabbit heart tissue, rat heart tissue, mouse heart tissue |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC detected in | SH-SY5Y cells, HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68532-2-Ig targets N-cadherin in WB, IF/ICC, FC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
| 经测试应用 | WB, IF/ICC, FC, ELISA Application Description |
| 经测试反应性 | human, mouse, rat, pig, rabbit |
| 免疫原 | Recombinant protein 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | cadherin 2, type 1, N-cadherin (neuronal) |
| 别名 | CDHN, CDH2, CD325, Cadherin-2, Cadherin 2 |
| 计算分子量 | 100 kDa |
| 观测分子量 | 130 kDa |
| GenBank蛋白编号 | NM_001792.4 |
| 基因名称 | N-cadherin |
| Gene ID (NCBI) | 1000 |
| RRID | AB_3670382 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P19022-1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. N-cadherin (neural cadherin), also known as CDH2 (cadherin 2), is a 130-kDa transmembrane protein and a classical member of the cadherin superfamily which also include E-, P-, R-, and B-cadherins. Expression of N-cadherin has been reported on various cell types including neurons, endothelial cells and cardiac myocytes (PMID: 11282032; 9508779; 8125202). N-cadherin has functions in early brain morphogenesis, synaptogenesis and synaptic plasticity (PMID: 23321619). The N-cadherin ectodomain can be cleaved, leading to the generation of a 90 kD N-terminal fragment (PMID: 16998833; 17028923).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for N-cadherin antibody 68532-2-Ig | Download protocol |
| IF protocol for N-cadherin antibody 68532-2-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
