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NAGS Recombinant antibody

NAGS Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

230279D3

Cat no : 82963-1-RR

Print datasheet

Synonyms

N-acetylglutamate synthase conserved domain form, EC:2.3.1.1, ARGA, Amino-acid acetyltransferase, AGAS

验证数据展示

  • WB analysis using 82963-1-RR

    WB analysis using 82963-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82963-1-RR (NAGS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 82963-1-RR

    Affinity and Kinetic Characterization of 82963-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82963-1-RR against Human NAGS were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHepG2 cells, mouse liver tissue, rat liver tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82963-1-RR targets NAGS in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen NAGS fusion protein Ag16073 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name N-acetylglutamate synthase
Synonyms N-acetylglutamate synthase conserved domain form, EC:2.3.1.1, ARGA, Amino-acid acetyltransferase, AGAS
Calculated Molecular Weight 534 aa, 58 kDa
Observed Molecular Weight 58 kDa
GenBank Accession NumberBC111713
Gene Symbol NAGS
Gene ID (NCBI) 162417
RRIDAB_3670709
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8N159
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

N-acetylglutamate synthase, also known as NAGS, is a mitochondrial enzyme that catalyzes the formation of N-acetylglutamate (NAG) from glutamate and acetyl coenzyme-A. NAGS is the key enzyme for regulating the hepatic urea cycle and is also highly expressed in the kidney and gut. Deficiency of NAGS leads to hyperammonemia due to decreased activity of CPSI deprived of its cofactor NAG(PMID:12459178,12754705).

实验方案

Product Specific Protocols
WB protocol for NAGS antibody 82963-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols