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验证数据展示

  • WB analysis using 66159-1-Ig (same clone as 66159-1-PBS)

    WB analysis using 66159-1-Ig (same clone as 66159-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66159-1-Ig (NAPRT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66159-1-PBS in a different storage buffer formulation.

  • WB analysis using 66159-1-Ig (same clone as 66159-1-PBS)

    WB analysis using 66159-1-Ig (same clone as 66159-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66159-1-Ig (NAPRT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66159-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 cells using 66159-1-Ig (same clone as 66159-1-PBS)

    WB analysis of HepG2 cells using 66159-1-Ig (same clone as 66159-1-PBS)

    WB result of NAPRT1 antibody (66159-1-Ig, 1:6000) with si-Control and si-NAPRT1 transfected HepG2 cells. This data was developed using the same antibody clone with 66159-1-PBS in a different storage buffer formulation.

  • IHC staining of human small intestine using 66159-1-Ig (same clone as 66159-1-PBS)

    IHC staining of human small intestine using 66159-1-Ig (same clone as 66159-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66159-1-Ig (NAPRT1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66159-1-PBS in a different storage buffer formulation.

  • IHC staining of human small intestine using 66159-1-Ig (same clone as 66159-1-PBS)

    IHC staining of human small intestine using 66159-1-Ig (same clone as 66159-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66159-1-Ig (NAPRT1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66159-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66159-1-Ig (same clone as 66159-1-PBS)

    IF Staining of HeLa using 66159-1-Ig (same clone as 66159-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 66159-1-Ig(NAPRT1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66159-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66159-1-Ig (same clone as 66159-1-PBS)

    IF Staining of HeLa using 66159-1-Ig (same clone as 66159-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using NAPRT1 antibody (66159-1-Ig, Clone: 5D8H10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66159-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

NAPRT1 Monoclonal antibody, PBS Only

NAPRT1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66159-1-PBS

产品说明书

CloneNo. 5D8H10

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

NAPRT, FHIP, FHA-HIT-interacting protein, FHA HIT interacting protein, EC:6.3.4.21

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66159-1-PBS targets NAPRT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 NAPRT1 fusion protein Ag4265 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 nicotinate phosphoribosyltransferase domain containing 1
别名 NAPRT, FHIP, FHA-HIT-interacting protein, FHA HIT interacting protein, EC:6.3.4.21
计算分子量 514 aa, 55 kDa
观测分子量 51 kDa
GenBank蛋白编号BC032466
基因名称 NAPRT1
Gene ID (NCBI) 93100
RRIDAB_2881555
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ6XQN6
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Nicotinic acid (NA) is a coenzyme in cellular redox reactions, and is an essential component of metabolic pathways in all living cells. NAPRT1 (Nicotinate phosphoribosyltransferase) is essential for increasing cellular NAD levels and, thus, to prevent oxidative stress of cells. NAPRT1 converts Nicotinic acid (NA; niacin) to NA mononucleotide (NaMN), which is then converted to NA adenine dinucleotide (NaAD), and finally to nicotinamide adenine dinucleotide (NAD).