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NAPRT1 Monoclonal antibody

NAPRT1 Monoclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

5D8H10

Cat No : 66159-1-Ig

Print datasheet

Synonyms

FHA HIT interacting protein, FHIP, NAPRT1, NAPRTase, PP3856

验证数据展示

  • WB analysis using 66159-1-Ig

    WB analysis using 66159-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66159-1-Ig (NAPRT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis using 66159-1-Ig

    WB analysis using 66159-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66159-1-Ig (NAPRT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 cells using 66159-1-Ig

    WB analysis of HepG2 cells using 66159-1-Ig

    WB result of NAPRT1 antibody (66159-1-Ig, 1:6000) with si-Control and si-NAPRT1 transfected HepG2 cells.

  • IHC staining of human small intestine using 66159-1-Ig

    IHC staining of human small intestine using 66159-1-Ig

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66159-1-Ig (NAPRT1 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human small intestine using 66159-1-Ig

    IHC staining of human small intestine using 66159-1-Ig

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66159-1-Ig (NAPRT1 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 66159-1-Ig

    IF Staining of HeLa using 66159-1-Ig

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 66159-1-Ig(NAPRT1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inTHP-1 cells, HEK-293 cells, Jurkat cells, HepG2 cells, LNCaP cells, HeLa cells, K-562 cells
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 4 publications below

产品信息

66159-1-Ig targets NAPRT1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, IHC, WB, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen NAPRT1 fusion protein Ag4265 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name nicotinate phosphoribosyltransferase domain containing 1
Synonyms FHA HIT interacting protein, FHIP, NAPRT1, NAPRTase, PP3856
Calculated Molecular Weight 514 aa, 55 kDa
Observed Molecular Weight 51 kDa
GenBank Accession NumberBC032466
Gene Symbol NAPRT1
Gene ID (NCBI) 93100
RRIDAB_2881555
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ6XQN6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nicotinic acid (NA) is a coenzyme in cellular redox reactions, and is an essential component of metabolic pathways in all living cells. NAPRT1 (Nicotinate phosphoribosyltransferase) is essential for increasing cellular NAD levels and, thus, to prevent oxidative stress of cells. NAPRT1 converts Nicotinic acid (NA; niacin) to NA mononucleotide (NaMN), which is then converted to NA adenine dinucleotide (NaAD), and finally to nicotinamide adenine dinucleotide (NAD).

实验方案

Product Specific Protocols
WB protocol for NAPRT1 antibody 66159-1-IgDownload protocol
IHC protocol for NAPRT1 antibody 66159-1-IgDownload protocol
IF protocol for NAPRT1 antibody 66159-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

Extracellular nicotinate phosphoribosyltransferase binds Toll like receptor 4 and mediates inflammation.

Authors - Antonella Managò
View Article
humanWB

Nat Commun

PPM1D mutations silence NAPRT gene expression and confer NAMPT inhibitor sensitivity in glioma.

Authors - Nathan R Fons
View Article
humanWB

Cell Chem Biol

NAMPT-targeting PROTAC and nicotinic acid co-administration elicit safe and robust anti-tumor efficacy in NAPRT-deficient pan-cancers

Authors - Xiaotong Zhu
View Article
humanWB

Eur J Med Chem

Discovery of potent and novel dual NAMPT/BRD4 inhibitors for efficient treatment of hepatocellular carcinoma

Authors - Chunjia Yin
View Article