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NAPRT1 Monoclonal antibody

NAPRT1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66159-1-Ig

产品说明书

CloneNo. 5D8H10

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

NAPRT, FHIP, FHA-HIT-interacting protein, FHA HIT interacting protein, EC:6.3.4.21

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inTHP-1 cells, HEK-293 cells, Jurkat cells, HepG2 cells, LNCaP cells, HeLa cells, K-562 cells
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:100-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 6 publications below

产品信息

66159-1-Ig targets NAPRT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human
免疫原 NAPRT1 fusion protein Ag4265 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 nicotinate phosphoribosyltransferase domain containing 1
别名 NAPRT, FHIP, FHA-HIT-interacting protein, FHA HIT interacting protein, EC:6.3.4.21
计算分子量 514 aa, 55 kDa
观测分子量 51 kDa
GenBank蛋白编号BC032466
基因名称 NAPRT1
Gene ID (NCBI) 93100
RRIDAB_2881555
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ6XQN6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nicotinic acid (NA) is a coenzyme in cellular redox reactions, and is an essential component of metabolic pathways in all living cells. NAPRT1 (Nicotinate phosphoribosyltransferase) is essential for increasing cellular NAD levels and, thus, to prevent oxidative stress of cells. NAPRT1 converts Nicotinic acid (NA; niacin) to NA mononucleotide (NaMN), which is then converted to NA adenine dinucleotide (NaAD), and finally to nicotinamide adenine dinucleotide (NAD).

实验方案

Product Specific Protocols
WB protocol for NAPRT1 antibody 66159-1-IgDownload protocol
IHC protocol for NAPRT1 antibody 66159-1-IgDownload protocol
IF protocol for NAPRT1 antibody 66159-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

Extracellular nicotinate phosphoribosyltransferase binds Toll like receptor 4 and mediates inflammation.

Authors - Antonella Managò
humanWB

Nat Commun

PPM1D mutations silence NAPRT gene expression and confer NAMPT inhibitor sensitivity in glioma.

Authors - Nathan R Fons
humanWB

Cell Chem Biol

NAMPT-targeting PROTAC and nicotinic acid co-administration elicit safe and robust anti-tumor efficacy in NAPRT-deficient pan-cancers

Authors - Xiaotong Zhu
humanWB

Mol Cancer Ther

Exploiting Metabolic Defects in Glioma with Nanoparticle Encapsulated NAMPT Inhibitors

Authors - Matthew A Murray
humanWB

Mol Cancer Res

NAPRT silencing in FH-deficient renal cell carcinoma confers therapeutic vulnerabilities via NAD+ depletion

Authors - Katelyn J Noronha
humanWB

Eur J Med Chem

Discovery of potent and novel dual NAMPT/BRD4 inhibitors for efficient treatment of hepatocellular carcinoma

Authors - Chunjia Yin
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