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验证数据展示

  • IF Staining of HeLa using CL488-66159

    IF Staining of HeLa using CL488-66159

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 NAPRT1 antibody (CL488-66159, Clone: 5D8H10 ) at dilution of 1:200.

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated NAPRT1 Monoclonal antibody

NAPRT1 Monoclonal Antibody for IF/ICC
Cat No. CL488-66159

Print datasheet

CloneNo. 5D8H10

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

IF/ICC

Synonyms

FHA HIT interacting protein, FHIP, NAPRT1, NAPRTase, PP3856

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66159-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66159 targets NAPRT1 in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse
Immunogen NAPRT1 fusion protein Ag4265 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name nicotinate phosphoribosyltransferase domain containing 1
Synonyms FHA HIT interacting protein, FHIP, NAPRT1, NAPRTase, PP3856
Calculated Molecular Weight 514 aa, 55 kDa
Observed Molecular Weight 51 kDa
GenBank Accession NumberBC032466
Gene Symbol NAPRT1
Gene ID (NCBI) 93100
RRIDAB_2883274
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ6XQN6
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nicotinic acid (NA) is a coenzyme in cellular redox reactions, and is an essential component of metabolic pathways in all living cells. NAPRT1 (Nicotinate phosphoribosyltransferase) is essential for increasing cellular NAD levels and, thus, to prevent oxidative stress of cells. NAPRT1 converts Nicotinic acid (NA; niacin) to NA mononucleotide (NaMN), which is then converted to NA adenine dinucleotide (NaAD), and finally to nicotinamide adenine dinucleotide (NAD).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 NAPRT1 antibody CL488-66159Download protocol
Standard Protocols
Click here to view our Standard Protocols