• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CoraLite®594-conjugated NARS Monoclonal antibody

NARS Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

4G5G12

Cat No : CL594-67711

Print datasheet

Synonyms

ASNRS, Asparagine tRNA ligase, asparaginyl tRNA synthetase, NARS, NARS1

验证数据展示

  • IF Staining of HeLa using CL594-67711

    IF Staining of HeLa using CL594-67711

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 NARS antibody (CL594-67711, Clone: 4G5G12 ) at dilution of 1:200.

经过测试的应用

Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy NARS Ready-To-Use IHC Kit. NARS primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67711-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67711 targets NARS in IF applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, mouse, rat
Immunogen NARS fusion protein Ag6656 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name asparaginyl-tRNA synthetase
Synonyms ASNRS, Asparagine tRNA ligase, asparaginyl tRNA synthetase, NARS, NARS1
Calculated Molecular Weight 63 kDa
Observed Molecular Weight63 kDa
GenBank Accession NumberBC001687
Gene Symbol NARS
Gene ID (NCBI) 4677
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO43776
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Asparagine-tRNA ligase (NARS) belongs to the class-II aminoacyl-tRNA synthetase family. Catalyzes the attachment of asparagine to tRNA(Asn) in a two-step reaction: asparagine is first activated by ATP to form Asn-AMP and then transferred to the acceptor end of tRNA(Asn) (PubMed: 9421509). In addition to its essential role in protein synthesis, acts as a signaling molecule that induced migration of CCR3-expressing cells. The NARS levels were higher in tumor tissues compared with adjacent normal tissues, and positively associated with LN metastasis. NARS may serve as a potential biomarker for lung adenocarcinoma (ADC) diagnosis/prognosis (PMID: 27161446).

实验方案

Product Specific Protocols
IF protocol for CL594 NARS antibody CL594-67711Download protocol
FC protocol for CL594 NARS antibody CL594-67711Download protocol
Standard Protocols
Click here to view our Standard Protocols