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验证数据展示

  • IF Staining of HeLa using CL594-67711

    IF Staining of HeLa using CL594-67711

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 NARS antibody (CL594-67711, Clone: 4G5G12 ) at dilution of 1:200.

  • FC experiment of HeLa using CL594-67711

    FC experiment of HeLa using CL594-67711

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human NARS (CL594-67711, Clone:4G5G12) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL594-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite®594-conjugated NARS Monoclonal antibody

NARS Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-67711

产品说明书

CloneNo. 4G5G12

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

Asparaginyl-tRNA synthetase 1, Asparaginyl-tRNA synthetase, asparaginyl tRNA synthetase, Asparagine--tRNA ligase, cytoplasmic, ASNRS

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy NARS Ready-To-Use IHC Kit. NARS primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67711-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67711 targets NARS in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 NARS fusion protein Ag6656 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 asparaginyl-tRNA synthetase
别名 Asparaginyl-tRNA synthetase 1, Asparaginyl-tRNA synthetase, asparaginyl tRNA synthetase, Asparagine--tRNA ligase, cytoplasmic, ASNRS
计算分子量 63 kDa
观测分子量63 kDa
GenBank蛋白编号BC001687
基因名称 NARS
Gene ID (NCBI) 4677
RRIDAB_2934775
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO43776
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Asparagine-tRNA ligase (NARS) belongs to the class-II aminoacyl-tRNA synthetase family. Catalyzes the attachment of asparagine to tRNA(Asn) in a two-step reaction: asparagine is first activated by ATP to form Asn-AMP and then transferred to the acceptor end of tRNA(Asn) (PubMed: 9421509). In addition to its essential role in protein synthesis, acts as a signaling molecule that induced migration of CCR3-expressing cells. The NARS levels were higher in tumor tissues compared with adjacent normal tissues, and positively associated with LN metastasis. NARS may serve as a potential biomarker for lung adenocarcinoma (ADC) diagnosis/prognosis (PMID: 27161446).

实验方案

Product Specific Protocols
IF protocol for CL594 NARS antibody CL594-67711Download protocol
FC protocol for CL594 NARS antibody CL594-67711Download protocol
Standard Protocols
Click here to view our Standard Protocols