• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

NAS1/SLC13A1 Polyclonal antibody

NAS1/SLC13A1 Polyclonal Antibody for WB, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 25039-1-AP

Print datasheet

Synonyms

SLC13A1, NAS1, hNaSi-1

验证数据展示

  • WB analysis of HEK-293 using 25039-1-AP

    WB analysis of HEK-293 using 25039-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25039-1-AP (NAS1/SLC13A1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse kidney using 25039-1-AP

    IF Staining of mouse kidney using 25039-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using NAS1/SLC13A1 antibody (25039-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse kidney using 25039-1-AP

    IF Staining of mouse kidney using 25039-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using NAS1/SLC13A1 antibody (25039-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, mouse kidney tissue
Positive IF-P detected inmouse kidney tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25039-1-AP targets NAS1/SLC13A1 in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF-P, ELISA Application Description
Tested Reactivity human, mouse
Immunogen NAS1/SLC13A1 fusion protein Ag19026 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 13 (sodium/sulfate symporters), member 1
Synonyms SLC13A1, NAS1, hNaSi-1
Calculated Molecular Weight 595 aa, 66 kDa
Observed Molecular Weight 66 kDa
GenBank Accession NumberBC148686
Gene Symbol NAS1
Gene ID (NCBI) 6561
RRIDAB_2879864
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BZW2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC13A1 also known as NAS1, is an apical membrane Na(+)-sulfate cotransporter involved in sulfate homeostasis in the kidney. SLC13A1 primarily participates in pH-independent, sodium-dependent cotransport of sulfate across renal proximal tubules and small intestinal epithelia(PMID: 38552027).

实验方案

Product Specific Protocols
WB protocol for NAS1/SLC13A1 antibody 25039-1-APDownload protocol
IF protocol for NAS1/SLC13A1 antibody 25039-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols