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验证数据展示

  • WB analysis of HEK-293 using 25039-1-AP

    WB analysis of HEK-293 using 25039-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25039-1-AP (NAS1/SLC13A1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse kidney using 25039-1-AP

    IF Staining of mouse kidney using 25039-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using NAS1/SLC13A1 antibody (25039-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse kidney using 25039-1-AP

    IF Staining of mouse kidney using 25039-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using NAS1/SLC13A1 antibody (25039-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NAS1/SLC13A1 Polyclonal antibody

NAS1/SLC13A1 Polyclonal Antibody for WB, IF-P, ELISA
Cat No. 25039-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF-P, ELISA

别名

SLC13A1, NAS1, hNaSi-1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, mouse kidney tissue
Positive IF-P detected inmouse kidney tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25039-1-AP targets NAS1/SLC13A1 in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原 NAS1/SLC13A1 fusion protein Ag19026 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 13 (sodium/sulfate symporters), member 1
别名 SLC13A1, NAS1, hNaSi-1
计算分子量 595 aa, 66 kDa
观测分子量 66 kDa
GenBank蛋白编号BC148686
基因名称 NAS1
Gene ID (NCBI) 6561
RRIDAB_2879864
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BZW2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC13A1 also known as NAS1, is an apical membrane Na(+)-sulfate cotransporter involved in sulfate homeostasis in the kidney. SLC13A1 primarily participates in pH-independent, sodium-dependent cotransport of sulfate across renal proximal tubules and small intestinal epithelia(PMID: 38552027).

实验方案

Product Specific Protocols
WB protocol for NAS1/SLC13A1 antibody 25039-1-APDownload protocol
IF protocol for NAS1/SLC13A1 antibody 25039-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols