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  • Featured Product
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NAT10 Monoclonal antibody

NAT10 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3E3D8

Cat no : 67465-1-Ig

Print datasheet

Synonyms

N acetyltransferase 10, KIAA1709, EC:2.3.1.-, ALP, 3E3D8

验证数据展示

  • WB analysis using 67465-1-Ig

    WB analysis using 67465-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67465-1-Ig (NAT10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 67465-1-Ig

    WB analysis of A549 using 67465-1-Ig

    WB result of NAT10 antibody (67465-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NAT10 transfected A549 cells.

经过测试的应用

Positive WB detected inLNCaP cells, A549 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, Neuro-2a cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67465-1-Ig targets NAT10 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen NAT10 fusion protein Ag4355 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name N-acetyltransferase 10 (GCN5-related)
Synonyms N acetyltransferase 10, KIAA1709, EC:2.3.1.-, ALP, 3E3D8
Calculated Molecular Weight 1025 aa, 116 kDa
Observed Molecular Weight 116 kDa
GenBank Accession NumberBC035558
Gene Symbol NAT10
Gene ID (NCBI) 55226
RRIDAB_2882694
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H0A0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NAT10 (N-acetyltransferase 10) is a nucleolar protein that is involved in regulation of telomerase activity, DNA damage response, and cytokinesis. It also plays a role in maintaining nuclear shape. Inhibition of NAT10 has been reported to rescue the misshapen nuclei in laminopathic cells via microtubule reorganization.

实验方案

Product Specific Protocols
WB protocol for NAT10 antibody 67465-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols