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验证数据展示

  • FC experiment of NIH/3T3 using CL488-67465

    FC experiment of NIH/3T3 using CL488-67465

    1x10^6 NIH/3T3 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Nat10 Monoclonal Antibody (CL488-67465, Clone:3E3D8)(red), or 0.8 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated NAT10 Monoclonal antibody

NAT10 Monoclonal Antibody for FC (Intra)
Cat No. CL488-67465

产品说明书

CloneNo. 3E3D8

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

FC (Intra)

别名

N acetyltransferase 10, KIAA1709, EC:2.3.1.-, ALP, 3E3D8

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inNIH/3T3 cells
For other applications, we recommend the unconjugated version of this antibody, 67465-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67465 targets NAT10 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 NAT10 fusion protein Ag4355 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 N-acetyltransferase 10 (GCN5-related)
别名 N acetyltransferase 10, KIAA1709, EC:2.3.1.-, ALP, 3E3D8
计算分子量 1025 aa, 116 kDa
观测分子量 116 kDa
GenBank蛋白编号BC035558
基因名称 NAT10
Gene ID (NCBI) 55226
RRIDAB_3672949
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9H0A0
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NAT10 (N-acetyltransferase 10) is a nucleolar protein that is involved in regulation of telomerase activity, DNA damage response, and cytokinesis. It also plays a role in maintaining nuclear shape. Inhibition of NAT10 has been reported to rescue the misshapen nuclei in laminopathic cells via microtubule reorganization.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 NAT10 antibody CL488-67465Download protocol
Standard Protocols
Click here to view our Standard Protocols