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验证数据展示

  • WB analysis using 82585-1-RR (same clone as 82585-1-PBS)

    WB analysis using 82585-1-RR (same clone as 82585-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82585-1-RR (NAT10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82585-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 82585-1-RR (same clone as 82585-1-PBS)

    WB analysis of HeLa using 82585-1-RR (same clone as 82585-1-PBS)

    WB result of NAT10 antibody (82585-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NAT10 transfected HeLa cells. This data was developed using the same antibody clone with 82585-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 82585-1-RR (same clone as 82585-1-PBS)

    IF Staining of HeLa using 82585-1-RR (same clone as 82585-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NAT10 antibody (82585-1-RR, Clone: 5B9 ) at dilution of 1:1900 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 82585-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse testis using 82585-1-RR (same clone as 82585-1-PBS)

    IP experiment of mouse testis using 82585-1-RR (same clone as 82585-1-PBS)

    IP result of anti-NAT10 (IP:82585-1-RR, 4ug; Detection:82585-1-RR 1:5000) with mouse testis tissue lysate 1120 ug. This data was developed using the same antibody clone with 82585-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 82585-1-RR (same clone as 82585-1-PBS)

    IF Staining of HeLa using 82585-1-RR (same clone as 82585-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NAT10 antibody (82585-1-RR, Clone: 5B9 ) at dilution of 1:1900 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 82585-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82585-1-RR

    Affinity and Kinetic Characterization of 82585-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82585-1-RR against Human NAT10 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

NAT10 Recombinant antibody, PBS Only

NAT10 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 82585-1-PBS

产品说明书

CloneNo. 5B9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, IP, Indirect ELISA

别名

N acetyltransferase 10, KIAA1709, EC:2.3.1.-, ALP, 5B9

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

82585-1-PBS targets NAT10 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原 NAT10 fusion protein Ag4184 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 N-acetyltransferase 10 (GCN5-related)
别名 N acetyltransferase 10, KIAA1709, EC:2.3.1.-, ALP, 5B9
计算分子量 1025 aa, 116 kDa
观测分子量 116 kDa
GenBank蛋白编号BC035558
基因名称 NAT10
Gene ID (NCBI) 55226
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9H0A0
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NAT10 (N-acetyltransferase 10) is a nucleolar protein that is involved in regulation of telomerase activity, DNA damage response, and cytokinesis. It also plays a role in maintaining nuclear shape. Inhibition of NAT10 has been reported to rescue the misshapen nuclei in laminopathic cells via microtubule reorganization. The specificity of this antibody has been tested by siRNA (PMID: 24786082). NAT10 regulates mitotic cell fate by acetylating Eg5. NAT10 depletion results in multinuclear giant cells, which is the hallmark of mitotic catastrophe (PMID: 35210604). NAT10 plays a crucial role in carcinogenesis through influencing EMT, hypoxia, ribosomal biogenesis and overall, promoting translational efficiency. Recently, we reported that treating cancer cells with Remodelin, a small molecule inhibitor of NAT10, causes alteration in global lipid metabolism (PMID: 36149760).