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验证数据展示

  • WB analysis using 82585-1-RR

    WB analysis using 82585-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82585-1-RR (NAT10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 82585-1-RR

    WB analysis of HeLa using 82585-1-RR

    WB result of NAT10 antibody (82585-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NAT10 transfected HeLa cells.

  • IF Staining of HeLa using 82585-1-RR

    IF Staining of HeLa using 82585-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NAT10 antibody (82585-1-RR, Clone: 5B9 ) at dilution of 1:1900 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • IP experiment of mouse testis using 82585-1-RR

    IP experiment of mouse testis using 82585-1-RR

    IP result of anti-NAT10 (IP:82585-1-RR, 4ug; Detection:82585-1-RR 1:5000) with mouse testis tissue lysate 1120 ug.

  • IF Staining of HeLa using 82585-1-RR

    IF Staining of HeLa using 82585-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NAT10 antibody (82585-1-RR, Clone: 5B9 ) at dilution of 1:1900 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

  • Affinity and Kinetic Characterization of 82585-1-RR

    Affinity and Kinetic Characterization of 82585-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82585-1-RR against Human NAT10 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

NAT10 Recombinant antibody

NAT10 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, IP, ELISA
Cat No. 82585-1-RR

产品说明书

CloneNo. 5B9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, IP, ELISA

别名

N acetyltransferase 10, KIAA1709, EC:2.3.1.-, ALP, 5B9

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, A549 cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IP detected inmouse testis tissue
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:1500-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82585-1-RR targets NAT10 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 human
免疫原 NAT10 fusion protein Ag4184 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 N-acetyltransferase 10 (GCN5-related)
别名 N acetyltransferase 10, KIAA1709, EC:2.3.1.-, ALP, 5B9
计算分子量 1025 aa, 116 kDa
观测分子量 116 kDa
GenBank蛋白编号BC035558
基因名称 NAT10
Gene ID (NCBI) 55226
RRIDAB_3086490
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9H0A0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NAT10 (N-acetyltransferase 10) is a nucleolar protein that is involved in regulation of telomerase activity, DNA damage response, and cytokinesis. It also plays a role in maintaining nuclear shape. Inhibition of NAT10 has been reported to rescue the misshapen nuclei in laminopathic cells via microtubule reorganization. The specificity of this antibody has been tested by siRNA (PMID: 24786082). NAT10 regulates mitotic cell fate by acetylating Eg5. NAT10 depletion results in multinuclear giant cells, which is the hallmark of mitotic catastrophe (PMID: 35210604). NAT10 plays a crucial role in carcinogenesis through influencing EMT, hypoxia, ribosomal biogenesis and overall, promoting translational efficiency. Recently, we reported that treating cancer cells with Remodelin, a small molecule inhibitor of NAT10, causes alteration in global lipid metabolism (PMID: 36149760).

实验方案

Product Specific Protocols
WB protocol for NAT10 antibody 82585-1-RRDownload protocol
IF protocol for NAT10 antibody 82585-1-RRDownload protocol
IP protocol for NAT10 antibody 82585-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols