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NAT10 Recombinant antibody

NAT10 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

5B9

Cat no : 82585-1-RR

Print datasheet

Synonyms

N acetyltransferase 10, KIAA1709, EC:2.3.1.-, ALP, 5B9

验证数据展示

  • WB analysis using 82585-1-RR

    WB analysis using 82585-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82585-1-RR (NAT10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 82585-1-RR

    IF Staining of HeLa using 82585-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NAT10 antibody (82585-1-RR, Clone: 5B9 ) at dilution of 1:1900 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • IP experiment of mouse testis using 82585-1-RR

    IP experiment of mouse testis using 82585-1-RR

    IP result of anti-NAT10 (IP:82585-1-RR, 4ug; Detection:82585-1-RR 1:5000) with mouse testis tissue lysate 1120 ug.

  • IF Staining of HeLa using 82585-1-RR

    IF Staining of HeLa using 82585-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NAT10 antibody (82585-1-RR, Clone: 5B9 ) at dilution of 1:1900 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

  • Affinity and Kinetic Characterization of 82585-1-RR

    Affinity and Kinetic Characterization of 82585-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82585-1-RR against Human NAT10 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHeLa cells, A549 cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IP detected inmouse testis tissue
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:1500-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82585-1-RR targets NAT10 in WB, IF/ICC, IP, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Tested Reactivity Human
Immunogen NAT10 fusion protein Ag4184 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name N-acetyltransferase 10 (GCN5-related)
Synonyms N acetyltransferase 10, KIAA1709, EC:2.3.1.-, ALP, 5B9
Calculated Molecular Weight 1025 aa, 116 kDa
Observed Molecular Weight 116 kDa
GenBank Accession NumberBC035558
Gene Symbol NAT10
Gene ID (NCBI) 55226
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H0A0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NAT10 (N-acetyltransferase 10) is a nucleolar protein that is involved in regulation of telomerase activity, DNA damage response, and cytokinesis. It also plays a role in maintaining nuclear shape. Inhibition of NAT10 has been reported to rescue the misshapen nuclei in laminopathic cells via microtubule reorganization. The specificity of this antibody has been tested by siRNA (PMID: 24786082). NAT10 regulates mitotic cell fate by acetylating Eg5. NAT10 depletion results in multinuclear giant cells, which is the hallmark of mitotic catastrophe (PMID: 35210604). NAT10 plays a crucial role in carcinogenesis through influencing EMT, hypoxia, ribosomal biogenesis and overall, promoting translational efficiency. Recently, we reported that treating cancer cells with Remodelin, a small molecule inhibitor of NAT10, causes alteration in global lipid metabolism (PMID: 36149760).

实验方案

Product Specific Protocols
WB protocol for NAT10 antibody 82585-1-RRDownload protocol
IF protocol for NAT10 antibody 82585-1-RRDownload protocol
IP protocol for NAT10 antibody 82585-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols