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NAT8L Polyclonal antibody

NAT8L Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 23841-1-AP

Print datasheet

Synonyms

NAA synthetase, Hcml3, EC:2.3.1.17, CML3, Camello-like protein 3

验证数据展示

  • WB analysis using 23841-1-AP

    WB analysis using 23841-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23841-1-AP (NAT8L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 23841-1-AP

    IF Staining of HEK-293 using 23841-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using NAT8L antibody (23841-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inHUVEC cells, mouse brain tissue, rat brain tissue
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

23841-1-AP targets NAT8L in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityrat
Immunogen NAT8L fusion protein Ag20832 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name N-acetyltransferase 8-like (GCN5-related, putative)
Synonyms NAA synthetase, Hcml3, EC:2.3.1.17, CML3, Camello-like protein 3
Calculated Molecular Weight 134 aa, 15 kDa
Observed Molecular Weight48 kDa
GenBank Accession NumberBC103748
Gene Symbol NAT8L
Gene ID (NCBI) 339983
RRIDAB_3669418
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ8N9F0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NAT8L (N-acetyltransferase 8-like) catalyzes the formation of N-acetyl aspartate (NAA) from acetyl-CoA and aspartate. In the brain, NAA delivers the acetate moiety to synthesize acetyl-CoA, which is further used for fatty acid generation (PMID: 24155240). NAT8L modulation may impinge on the metabolic reprogramming of cancer cells (PMID: 36580805). The calculated MW of NAT8L is around 33 kDa, and the observed MW is around 45 kDa.

实验方案

Product Specific Protocols
WB protocol for NAT8L antibody 23841-1-APDownload protocol
IF protocol for NAT8L antibody 23841-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Naunyn Schmiedebergs Arch Pharmacol

Molecular mechanism of geniposide against ANIT-induced intrahepatic cholestasis by integrative analysis of transcriptomics and metabolomics

Authors - Junyi Zhang
View Article