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NAT8L Polyclonal antibody

NAT8L Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 23841-1-AP

Print datasheet

Synonyms

NAA synthetase, Hcml3, EC:2.3.1.17, CML3, Camello-like protein 3

验证数据展示

  • WB analysis using 23841-1-AP

    WB analysis using 23841-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23841-1-AP (NAT8L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 23841-1-AP

    IF Staining of HEK-293 using 23841-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using NAT8L antibody (23841-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inHUVEC cells, mouse brain tissue, rat brain tissue
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

23841-1-AP targets NAT8L in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityrat
Immunogen NAT8L fusion protein Ag20832 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name N-acetyltransferase 8-like (GCN5-related, putative)
Synonyms NAA synthetase, Hcml3, EC:2.3.1.17, CML3, Camello-like protein 3
Calculated Molecular Weight 134 aa, 15 kDa
Observed Molecular Weight48 kDa
GenBank Accession NumberBC103748
Gene Symbol NAT8L
Gene ID (NCBI) 339983
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ8N9F0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NAT8L (N-acetyltransferase 8-like) catalyzes the formation of N-acetyl aspartate (NAA) from acetyl-CoA and aspartate. In the brain, NAA delivers the acetate moiety to synthesize acetyl-CoA, which is further used for fatty acid generation (PMID: 24155240). NAT8L modulation may impinge on the metabolic reprogramming of cancer cells (PMID: 36580805). The calculated MW of NAT8L is around 33 kDa, and the observed MW is around 45 kDa.

实验方案

Product Specific Protocols
WB protocol for NAT8L antibody 23841-1-APDownload protocol
IF protocol for NAT8L antibody 23841-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Naunyn Schmiedebergs Arch Pharmacol

Molecular mechanism of geniposide against ANIT-induced intrahepatic cholestasis by integrative analysis of transcriptomics and metabolomics

Authors - Junyi Zhang
View Article