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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated NBR1 Polyclonal antibody

NBR1 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-16004

Print datasheet

Synonyms

MIG19, M17S2, FLJ55359, Cell migration-inducing gene 19 protein, 1A13B

验证数据展示

  • FC experiment of HeLa using CL488-16004

    FC experiment of HeLa using CL488-16004

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human NBR1 (CL488-16004) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HeLa using CL488-16004

    IF Staining of HeLa using CL488-16004

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 NBR1 antibody (CL488-16004) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 16004-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-16004 targets NBR1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat, monkey
Immunogen NBR1 fusion protein Ag8652 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name neighbor of BRCA1 gene 1
Synonyms MIG19, M17S2, FLJ55359, Cell migration-inducing gene 19 protein, 1A13B
Calculated Molecular Weight 966 aa, 107 kDa
Observed Molecular Weight 140 kDa
GenBank Accession NumberBC009808
Gene Symbol NBR1
Gene ID (NCBI) 4077
RRIDAB_2919128
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14596
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NBR1, also named as 1A13B, KIAA0049 and M17S2, acts probably as a receptor for selective autophagosomal degradation of ubiquitinated targets. NBR1 and P62 can bind to autophagic effector proteins (Atg8 in yeast, MAP1LC3 protein family in mammals) anchored in the membrane of autophagosomes. It is a highly conserved multidomain scaffold protein with proposed roles in endocytic trafficking and selective autophagy. NBR1 is a novel PB1 adapter in Th2 differentiation and asthma. It functions as an autophagy receptor involved in targeting ubiquitinated proteins for degradation. It also has a dual role as a scaffold protein to regulate growth-factor receptor and downstream signaling pathways. Observed MW of NBR1 is 140 kDa (PMID: 22654911, PMID: 22484440).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 NBR1 antibody CL488-16004Download protocol
FC protocol for CL Plus 488 NBR1 antibody CL488-16004Download protocol
Standard Protocols
Click here to view our Standard Protocols