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  • Featured Product
  • KD/KO Validated

NCAM1/CD56 Recombinant antibody, PBS Only

NCAM1/CD56 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

6N11

Cat no : 82715-1-PBS

Print datasheet

Synonyms

CD56, NCAM1, 6N11, NCAM 1, NCAM

验证数据展示

  • WB analysis of U-251 using 82715-1-RR (same clone as 82715-1-PBS)

    WB analysis of U-251 using 82715-1-RR (same clone as 82715-1-PBS)

    WB result of NCAM1/CD56 antibody (82715-1-RR; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NCAM1/CD56 transfected U-251 cells. This data was developed using the same antibody clone with 82715-1-PBS in a different storage buffer formulation.

  • WB analysis using 82715-1-RR (same clone as 82715-1-PBS)

    WB analysis using 82715-1-RR (same clone as 82715-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82715-1-RR (NCAM1/CD56 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82715-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 82715-1-RR (same clone as 82715-1-PBS)

    IHC staining of mouse brain using 82715-1-RR (same clone as 82715-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82715-1-RR (NCAM1/CD56 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82715-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD56/NCAM1 antibody included
  • IHC staining of mouse brain using 82715-1-RR (same clone as 82715-1-PBS)

    IHC staining of mouse brain using 82715-1-RR (same clone as 82715-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82715-1-RR (NCAM1/CD56 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82715-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD56/NCAM1 antibody included
  • IF Staining of SH-SY5Y using 82715-1-RR (same clone as 82715-1-PBS)

    IF Staining of SH-SY5Y using 82715-1-RR (same clone as 82715-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using NCAM1/CD56 antibody (82715-1-RR, Clone: 6N11 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82715-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82715-1-RR

    Affinity and Kinetic Characterization of 82715-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82715-1-RR against Human NCAM1 were performed. The affinity constant is 0.23 nM.

Planning an IHC experiment? We recommend our IHCeasy CD56/NCAM1 Ready-To-Use IHC Kit. CD56/NCAM1 primary antibody included.

产品信息

82715-1-PBS targets NCAM1/CD56 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen NCAM1/CD56 fusion protein Ag5528 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name neural cell adhesion molecule 1
Synonyms CD56, NCAM1, 6N11, NCAM 1, NCAM
Calculated Molecular Weight 95 kDa
Observed Molecular Weight 120 kDa, 140 kDa, 180 kDa
GenBank Accession NumberBC047244
Gene Symbol NCAM1
Gene ID (NCBI) 4684
ENSEMBL Gene IDENSG00000149294
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP13591
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Neural cell adhesion molecule 1 (NCAM1, also known as CD56) is a cell adhesion glycoprotein of the immunoglobulin (Ig) superfamily. It is a multifunction protein involved in synaptic plasticity, neurodevelopment, and neurogenesis. NCAM1 is expressed on human neurons, glial cells, skeletal muscle cells, NK cells and a subset of T cells, and the expression is observed in a wide variety of human tumors, including myeloma, myeloid leukemia, neuroendocrine tumors, Wilms' tumor, neuroblastoma, and NK/T cell lymphomas. Three major isoforms of NCAM1, with molecular masses of 120, 140, and 180 kDa, are generated by alternative splicing of mRNA (PMID: 9696812). The glycosylphosphatidylinositol (GPI)-anchored NCAM120 and the transmembrane NCAM140 and NCAM180 consist of five Ig-like domains and two fibronection-type III repeats (FNIII). All three forms can be posttranslationally modified by addition of polysialic acid (PSA) (PMID: 14976519). Several other isofroms have also been described (PMID: 1856291).