验证数据展示
经过测试的应用
Positive WB detected in | pig brain tissue, SH-SY5Y cells, U-251 cells, Neuro-2a cells, C6 cells, mouse brain tissue, rat brain tissue |
Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:200-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IHC | See 1 publications below |
产品信息
82715-1-RR targets NCAM1/CD56 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | IHC |
经测试反应性 | human, mouse, rat, pig |
文献引用反应性 | human |
免疫原 | NCAM1/CD56 fusion protein Ag5528 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | neural cell adhesion molecule 1 |
别名 | CD56, NCAM1, 6N11, MSK39, N CAM 1 |
计算分子量 | 95 kDa |
观测分子量 | 120 kDa, 140 kDa, 180 kDa |
GenBank蛋白编号 | BC047244 |
基因名称 | NCAM1 |
Gene ID (NCBI) | 4684 |
ENSEMBL Gene ID | ENSG00000149294 |
RRID | AB_3086516 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P13591 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Neural cell adhesion molecule 1 (NCAM1, also known as CD56) is a cell adhesion glycoprotein of the immunoglobulin (Ig) superfamily. It is a multifunction protein involved in synaptic plasticity, neurodevelopment, and neurogenesis. NCAM1 is expressed on human neurons, glial cells, skeletal muscle cells, NK cells and a subset of T cells, and the expression is observed in a wide variety of human tumors, including myeloma, myeloid leukemia, neuroendocrine tumors, Wilms' tumor, neuroblastoma, and NK/T cell lymphomas. Three major isoforms of NCAM1, with molecular masses of 120, 140, and 180 kDa, are generated by alternative splicing of mRNA (PMID: 9696812). The glycosylphosphatidylinositol (GPI)-anchored NCAM120 and the transmembrane NCAM140 and NCAM180 consist of five Ig-like domains and two fibronection-type III repeats (FNIII). All three forms can be posttranslationally modified by addition of polysialic acid (PSA) (PMID: 14976519). Several other isofroms have also been described (PMID: 1856291).
实验方案
Product Specific Protocols | |
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WB protocol for NCAM1/CD56 antibody 82715-1-RR | Download protocol |
IHC protocol for NCAM1/CD56 antibody 82715-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |