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验证数据展示

  • IF Staining of mouse brain using CL488-82715

    IF Staining of mouse brain using CL488-82715

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CoraLite® Plus 488 NCAM1/CD56 antibody (CL488-82715, Clone: 6N11 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using CL488-82715

    IF Staining of mouse brain using CL488-82715

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CoraLite® Plus 488 NCAM1/CD56 antibody (CL488-82715, Clone: 6N11 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated NCAM1/CD56 Recombinant antibody

NCAM1/CD56 Uni-rAbTM Recombinant Antibody for IF-P
Cat No. CL488-82715

产品说明书

CloneNo. 6N11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig

应用

IF-P

别名

CD56, NCAM1, 6N11, NCAM 1, NCAM

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse brain tissue
Planning an IHC experiment? We recommend our IHCeasy NCAM1/CD56 Ready-To-Use IHC Kit. NCAM1/CD56 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 82715-1-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-82715 targets NCAM1/CD56 in IF-P applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 IF-P Application Description
经测试反应性 human, mouse, rat, pig
免疫原 NCAM1/CD56 fusion protein Ag5528 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 neural cell adhesion molecule 1
别名 CD56, NCAM1, 6N11, NCAM 1, NCAM
计算分子量 95 kDa
观测分子量 120 kDa, 140 kDa, 180 kDa
GenBank蛋白编号BC047244
基因名称 NCAM1
Gene ID (NCBI) 4684
ENSEMBL Gene IDENSG00000149294
RRIDAB_3673074
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP13591
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Neural cell adhesion molecule 1 (NCAM1, also known as CD56) is a cell adhesion glycoprotein of the immunoglobulin (Ig) superfamily. It is a multifunction protein involved in synaptic plasticity, neurodevelopment, and neurogenesis. NCAM1 is expressed on human neurons, glial cells, skeletal muscle cells, NK cells and a subset of T cells, and the expression is observed in a wide variety of human tumors, including myeloma, myeloid leukemia, neuroendocrine tumors, Wilms' tumor, neuroblastoma, and NK/T cell lymphomas. Three major isoforms of NCAM1, with molecular masses of 120, 140, and 180 kDa, are generated by alternative splicing of mRNA (PMID: 9696812). The glycosylphosphatidylinositol (GPI)-anchored NCAM120 and the transmembrane NCAM140 and NCAM180 consist of five Ig-like domains and two fibronection-type III repeats (FNIII). All three forms can be posttranslationally modified by addition of polysialic acid (PSA) (PMID: 14976519). Several other isofroms have also been described (PMID: 1856291).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 NCAM1/CD56 antibody CL488-82715Download protocol
Standard Protocols
Click here to view our Standard Protocols