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Host
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验证数据展示

  • WB analysis of SH-SY5Y using 83365-6-RR (same clone as 83365-6-PBS)

    WB analysis of SH-SY5Y using 83365-6-RR (same clone as 83365-6-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 83365-6-RR (NCAM1/CD56 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83365-6-PBS in a different storage buffer formulation.

  • IF Staining of SH-SY5Y using 83365-6-RR (same clone as 83365-6-PBS)

    IF Staining of SH-SY5Y using 83365-6-RR (same clone as 83365-6-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using NCAM1/CD56 antibody (83365-6-RR, Clone: 240078C9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83365-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83365-6-RR

    Affinity and Kinetic Characterization of 83365-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83365-6-RR against Human NCAM1/CD56 were performed. The affinity constant is 3.45 nM.

NCAM1/CD56 Recombinant antibody, PBS Only

NCAM1/CD56 Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 83365-6-PBS

产品说明书

CloneNo. 240078C9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

CD56, NCAM1, 240078C9, NCAM 1, NCAM

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy NCAM1/CD56 Ready-To-Use IHC Kit. NCAM1/CD56 primary antibody included.

产品信息

83365-6-PBS targets NCAM1/CD56 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 neural cell adhesion molecule 1
别名 CD56, NCAM1, 240078C9, NCAM 1, NCAM
计算分子量 95 kDa
观测分子量160 kDa
GenBank蛋白编号BC047244
基因名称 NCAM1
Gene ID (NCBI) 4684
ENSEMBL Gene IDENSG00000149294
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP13591
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Neural cell adhesion molecule 1 (NCAM1, also known as CD56) is a cell adhesion glycoprotein of the immunoglobulin (Ig) superfamily. It is a multifunction protein involved in synaptic plasticity, neurodevelopment, and neurogenesis. NCAM1 is expressed on human neurons, glial cells, skeletal muscle cells, NK cells and a subset of T cells, and the expression is observed in a wide variety of human tumors, including myeloma, myeloid leukemia, neuroendocrine tumors, Wilms' tumor, neuroblastoma, and NK/T cell lymphomas. Three major isoforms of NCAM1, with molecular masses of 120, 140, and 180 kDa, are generated by alternative splicing of mRNA (PMID: 9696812). The glycosylphosphatidylinositol (GPI)-anchored NCAM120 and the transmembrane NCAM140 and NCAM180 consist of five Ig-like domains and two fibronection-type III repeats (FNIII). All three forms can be posttranslationally modified by addition of polysialic acid (PSA) (PMID: 14976519). Several other isofroms have also been described (PMID: 1856291).