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验证数据展示

  • WB analysis using 83494-6-RR

    WB analysis using 83494-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83494-6-RR (NCAM2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse brain using 83494-6-RR

    IF Staining of mouse brain using 83494-6-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using NCAM2 antibody (83494-6-RR, Clone: 240398D9 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83494-6-RR

    Affinity and Kinetic Characterization of 83494-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83494-6-RR against Human NCAM2 were performed. The affinity constant is 4.48 nM.

NCAM2 Recombinant antibody

NCAM2 Recombinant Antibody for WB, IF-P, ELISA
Cat No. 83494-6-RR

产品说明书

CloneNo. 240398D9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF-P, ELISA

别名

NCAM21, NCAM-2, NCAM 2, N CAM 2, 240398D9

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, mouse brain tissue
Positive IF-P detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)-PIF-P : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83494-6-RR targets NCAM2 in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原 NCAM2 fusion protein Ag4821 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 neural cell adhesion molecule 2
别名 NCAM21, NCAM-2, NCAM 2, N CAM 2, 240398D9
计算分子量 93 kDa
观测分子量125 kDa
GenBank蛋白编号BC052946
基因名称 NCAM2
Gene ID (NCBI) 4685
RRIDAB_3671121
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO15394
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The mammalian NCAM family has two members, NCAM1 and NCAM2 (PMID: 9729879; 20439718). Neural cell adhesion molecule 2 (NCAM2, also known as NCAM21) is a single-pass type I membrane protein that is highly expressed in the brain, which may play important roles in selective fasciculation and zone-to-zone projection of the primary olfactory axons or in neurodegenerative diseases such as Alzheimer's disease (PMID: 26611261). NCAM2 has two isoforms: NCAM2.1 bearing transmembrane and cytoplasmic domains and the shorter NCAM2.2 isoform, which is GPI-anchored (PMID: 9334170; 9221781). The molecular weight of NCAM2 is around 125 kDa and 93 kDa (PMID: 9334170; 3171685).

实验方案

Product Specific Protocols
WB protocol for NCAM2 antibody 83494-6-RRDownload protocol
IF protocol for NCAM2 antibody 83494-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols