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Host
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0
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0
Conjugate
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验证数据展示

  • WB analysis of HepG2 using 16828-1-AP (same clone as 16828-1-PBS)

    WB analysis of HepG2 using 16828-1-AP (same clone as 16828-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16828-1-AP (NCAPD3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16828-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 16828-1-AP (same clone as 16828-1-PBS)

    IP experiment of HeLa using 16828-1-AP (same clone as 16828-1-PBS)

    IP result of anti-NCAPD3 (IP:16828-1-AP, 4ug; Detection:16828-1-AP 1:500) with HeLa cells lysate 600ug. This data was developed using the same antibody clone with 16828-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 16828-1-AP (same clone as 16828-1-PBS)

    IF Staining of HepG2 using 16828-1-AP (same clone as 16828-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 16828-1-AP (NCAPD3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16828-1-PBS in a different storage buffer formulation.

  • WB analysis using 16828-1-AP (same clone as 16828-1-PBS)

    WB analysis using 16828-1-AP (same clone as 16828-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16828-1-AP (NCAPD3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16828-1-PBS in a different storage buffer formulation.

  • WB analysis using 16828-1-AP (same clone as 16828-1-PBS)

    WB analysis using 16828-1-AP (same clone as 16828-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16828-1-AP (NCAPD3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16828-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

NCAPD3 Polyclonal antibody, PBS Only

NCAPD3 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 16828-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

16828-1-PBS targets NCAPD3 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原 NCAPD3 fusion protein Ag10429 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 non-SMC condensin II complex, subunit D3
别名
计算分子量 1498 aa, 169 kDa
观测分子量 165 kDa
GenBank蛋白编号BC011408
基因名称 NCAPD3
Gene ID (NCBI) 23310
RRIDAB_2282528
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP42695
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Condensin complexes I and II play essential roles in mitotic chromosome assembly and segregation. Both condensins contain 2 invariant structural maintenance of chromosome (SMC) subunits, SMC2 and SMC4 , but they contain different sets of non-SMC subunits. NCAPD3 is 1 of 3 non-SMC subunits that define condensin II ([PMID: 14532007).