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验证数据展示

  • WB analysis using 68082-1-Ig

    WB analysis using 68082-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68082-1-Ig (NCAPG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control.

  • IF Staining of U2OS using 68082-1-Ig

    IF Staining of U2OS using 68082-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using NCAPG antibody (68082-1-Ig, Clone: 2D4D3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • FC experiment of U2OS using 68082-1-Ig

    FC experiment of U2OS using 68082-1-Ig

    1X10^6 U2OS cells were intracellularly stained with 0.4 ug Anti-Human NCAPG (68082-1-Ig, Clone:2D4D3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

NCAPG Monoclonal antibody

NCAPG Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 68082-1-Ig

产品说明书

CloneNo. 2D4D3

宿主/亚型

Mouse / IgG2b

种属反应性

Human

应用

WB, IF/ICC, FC (Intra), ELISA

别名

CAPG, CHCG, Condensin complex subunit 3, Condensin subunit CAP G, FLJ12450, HCAP G, Melanoma antigen NY MEL 3, NCAPG, NY MEL 3, NYMEL3, XCAP G homolog

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inU2OS cells
Positive FC detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy CAPG Ready-To-Use IHC Kit. CAPG primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68082-1-Ig targets NCAPG in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 Human
免疫原 NCAPG fusion protein Ag19926 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 non-SMC condensin I complex, subunit G
别名 CAPG, CHCG, Condensin complex subunit 3, Condensin subunit CAP G, FLJ12450, HCAP G, Melanoma antigen NY MEL 3, NCAPG, NY MEL 3, NYMEL3, XCAP G homolog
计算分子量 1015 aa, 114 kDa
观测分子量114 kDa
GenBank蛋白编号BC068467
基因名称 NCAPG
Gene ID (NCBI) 64151
RRIDAB_2918819
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9BPX3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NCAPG antibody 68082-1-IgDownload protocol
IF protocol for NCAPG antibody 68082-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols