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验证数据展示

  • IF Staining of U2OS using CL488-68082

    IF Staining of U2OS using CL488-68082

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CoraLite® Plus 488 NCAPG antibody (CL488-68082, Clone: 2D4D3 ) at dilution of 1:200.

  • FC experiment of U2OS using CL488-68082

    FC experiment of U2OS using CL488-68082

    1X10^6 U2OS cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human NCAPG (CL488-68082, Clone:2D4D3) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated NCAPG Monoclonal antibody

NCAPG Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-68082

产品说明书

CloneNo. 2D4D3

宿主/亚型

Mouse / IgG2b

种属反应性

Human

应用

IF/ICC, FC (Intra)

别名

CAPG, CHCG, Condensin complex subunit 3, Condensin subunit CAP G, FLJ12450, HCAP G, Melanoma antigen NY MEL 3, NCAPG, NY MEL 3, NYMEL3, XCAP G homolog

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inU2OS cells
Positive FC detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy CAPG Ready-To-Use IHC Kit. CAPG primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68082-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68082 targets NCAPG in IF/ICC, FC (Intra) applications and shows reactivity with Human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 Human
免疫原 NCAPG fusion protein Ag19926 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 non-SMC condensin I complex, subunit G
别名 CAPG, CHCG, Condensin complex subunit 3, Condensin subunit CAP G, FLJ12450, HCAP G, Melanoma antigen NY MEL 3, NCAPG, NY MEL 3, NYMEL3, XCAP G homolog
计算分子量 1015 aa, 114 kDa
观测分子量114 kDa
GenBank蛋白编号BC068467
基因名称 NCAPG
Gene ID (NCBI) 64151
RRIDAB_2923880
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9BPX3
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 NCAPG antibody CL488-68082Download protocol
FC protocol for CL Plus 488 NCAPG antibody CL488-68082Download protocol
Standard Protocols
Click here to view our Standard Protocols