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NOXA2/p67phox Monoclonal antibody

NOXA2/p67phox Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1D11A3

Cat no : 67594-1-Ig

Print datasheet

Synonyms

FLJ93058, NADPH oxidase activator 2, NCF 2, NCF2, Neutrophil cytosol factor 2, neutrophil cytosolic factor 2, NOXA2, P67 PHOX, P67PHOX

验证数据展示

  • WB analysis of HepG2 using 67594-1-Ig

    WB analysis of HepG2 using 67594-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67594-1-Ig (NOXA2/p67phox antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of L02 using 67594-1-Ig

    WB analysis of L02 using 67594-1-Ig

    L02 cells were subjected to SDS PAGE followed by western blot with 67594-1-Ig (NOXA2/p67phox antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 67594-1-Ig

    IHC staining of human tonsillitis using 67594-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67594-1-Ig (NOXA2/p67phox antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NCF2 antibody included
  • IF Staining of A431 using 67594-1-Ig

    IF Staining of A431 using 67594-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using NOXA2/p67phox antibody (67594-1-Ig, Clone: 1D11A3 ) at dilution of 1:800 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inHepG2 cells, L02 cells, pig liver tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy NCF2 Ready-To-Use IHC Kit. NCF2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67594-1-Ig targets NOXA2/p67phox in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, pig samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human, pig
Immunogen NOXA2/p67phox fusion protein Ag8691 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name neutrophil cytosolic factor 2
Synonyms FLJ93058, NADPH oxidase activator 2, NCF 2, NCF2, Neutrophil cytosol factor 2, neutrophil cytosolic factor 2, NOXA2, P67 PHOX, P67PHOX
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 67 kDa
GenBank Accession NumberBC001606
Gene Symbol NCF2
Gene ID (NCBI) 4688
RRIDAB_2882801
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP19878
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NOXA2/p67phox antibody 67594-1-IgDownload protocol
IHC protocol for NOXA2/p67phox antibody 67594-1-IgDownload protocol
IF protocol for NOXA2/p67phox antibody 67594-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols