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验证数据展示

  • IF Staining of A431 using CL488-67594

    IF Staining of A431 using CL488-67594

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite® Plus 488 NOXA2/p67phox antibody (CL488-67594, Clone: 1D11A3 ) at dilution of 1:1000.

CoraLite® Plus 488-conjugated NOXA2/p67phox Monoclonal antibody

NOXA2/p67phox Monoclonal Antibody for IF/ICC
Cat No. CL488-67594

产品说明书

CloneNo. 1D11A3

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

IF/ICC

别名

FLJ93058, NADPH oxidase activator 2, NCF 2, NCF2, Neutrophil cytosol factor 2, neutrophil cytosolic factor 2, NOXA2, P67 PHOX, P67PHOX

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy NCF2 Ready-To-Use IHC Kit. NCF2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67594-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67594 targets NOXA2/p67phox in IF/ICC applications and shows reactivity with Human samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human
免疫原 NOXA2/p67phox fusion protein Ag8691 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 neutrophil cytosolic factor 2
别名 FLJ93058, NADPH oxidase activator 2, NCF 2, NCF2, Neutrophil cytosol factor 2, neutrophil cytosolic factor 2, NOXA2, P67 PHOX, P67PHOX
计算分子量 60 kDa
观测分子量 67 kDa
GenBank蛋白编号BC001606
基因名称 NCF2
Gene ID (NCBI) 4688
RRIDAB_3084362
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP19878
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NOXA2/p67phox is also named as NCF2(Neutrophil cytosol factor 2) and belongs to the NCF2/NOXA1 family. It is a membrane bound cytochrome b558 required for activation of the latent NADPH oxidase. NCF2 is an essential component of the multi-protein NADPH oxidase enzyme in phagocytic leukocytes, as well as in certain non-phagocytic cells. This protein has 4 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 NOXA2/p67phox antibody CL488-67594Download protocol
Standard Protocols
Click here to view our Standard Protocols