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验证数据展示

  • IF Staining of A431 using CL488-67594

    IF Staining of A431 using CL488-67594

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite® Plus 488 NOXA2/p67phox antibody (CL488-67594, Clone: 1D11A3 ) at dilution of 1:1000.

CoraLite® Plus 488-conjugated NOXA2/p67phox Monoclonal antibody

NOXA2/p67phox Monoclonal Antibody for IF/ICC
Cat No. CL488-67594

Print datasheet

CloneNo. 1D11A3

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC

Synonyms

FLJ93058, NADPH oxidase activator 2, NCF 2, NCF2, Neutrophil cytosol factor 2, neutrophil cytosolic factor 2, NOXA2, P67 PHOX, P67PHOX

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy NCF2 Ready-To-Use IHC Kit. NCF2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67594-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67594 targets NOXA2/p67phox in IF/ICC applications and shows reactivity with Human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human
Immunogen NOXA2/p67phox fusion protein Ag8691 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name neutrophil cytosolic factor 2
Synonyms FLJ93058, NADPH oxidase activator 2, NCF 2, NCF2, Neutrophil cytosol factor 2, neutrophil cytosolic factor 2, NOXA2, P67 PHOX, P67PHOX
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 67 kDa
GenBank Accession NumberBC001606
Gene Symbol NCF2
Gene ID (NCBI) 4688
RRIDAB_3084362
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP19878
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NOXA2/p67phox is also named as NCF2(Neutrophil cytosol factor 2) and belongs to the NCF2/NOXA1 family. It is a membrane bound cytochrome b558 required for activation of the latent NADPH oxidase. NCF2 is an essential component of the multi-protein NADPH oxidase enzyme in phagocytic leukocytes, as well as in certain non-phagocytic cells. This protein has 4 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 NOXA2/p67phox antibody CL488-67594Download protocol
Standard Protocols
Click here to view our Standard Protocols